Anti-KDM2B Polyclonal Antibody (KD Validated) for WB and ELISA - Q8NHM5

Core Product Specifications and Parameters

Biological Background: KDM2B Function and Localization

• KDM2B is a histone demethylase that specifically demethylates 'Lys-4' and 'Lys-36' of histone H3, with preference for trimethylated H3K4 and dimethylated H3K36.
  Related references: PMID:16362057 PMID:17994099 PMID:26237645
• It binds to the transcribed region of ribosomal RNA and represses rRNA transcription, thereby inhibiting cell growth and proliferation.
  Related references: PMID:16362057 PMID:17994099
• KDM2B may function as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex (probable).
• The protein localizes to the nucleus and nucleolus, and associates with chromosomes.
• KDM2B contains a CXXC zinc finger, a JmjC domain, an F-box, and leucine-rich repeats, contributing to its roles in chromatin regulation, DNA/RNA binding, and ubiquitin conjugation.
• Alternative splicing produces multiple isoforms, and mutations are associated with intellectual disability, as reflected in UniProt keywords.
• Post-translational modifications include phosphorylation and ubiquitination, as indicated by phosphoprotein and Ubl conjugation keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1059-1336) of human KDM2B. When validating antibody specificity, consider using a lysate from cells with high KDM2B expression or an overexpression control.
• For Western blot, the predicted molecular weight is approximately 153 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In ELISA, use the recombinant protein as a positive control to establish a standard curve and assess linearity.
• Given KDM2B’s nuclear localization, nuclear extracts may be more suitable than whole-cell lysates for detection.
• Cross-reactivity is indicated for human; reactivity with other species should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material partner, CamelBio bridges the gap from concept to clinic. Alongside KDM2B polyclonal antibodies, we provide validated antibody pairs, optimized monoclonals, and bulk ancillary reagents for epigenetic biomarker development. Whether you’re developing chromatin-modifying enzyme assays or sourcing rare-target detection materials, CamelBio streamlines your procurement with end-to-end technical support.