Anti-KDM2B Polyclonal Antibody for WB and ELISA - Q8NHM5

Core Product Specifications and Parameters

Biological Background: KDM2B Function and Localization

• Lysine-specific demethylase 2B (KDM2B), also known as CXXC2, FBXL10, JHDM1B, and PCCX2, is encoded by the KDM2B gene in humans. It belongs to the JmjC domain-containing histone demethylase family.
• KDM2B functions as a histone demethylase that specifically removes methyl groups from 'Lys-4' and 'Lys-36' of histone H3, with a preference for tri-methylated H3K4 and di-methylated H3K36 substrates. This activity plays a central role in the histone code. Related references: PMID:16362057 PMID:17994099 PMID:26237645
• By preferentially binding to the transcribed regions of ribosomal RNA genes, KDM2B represses their transcription, leading to inhibited cell growth and proliferation. Related references: PMID:16362057 PMID:17994099
• KDM2B may also function as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, suggesting a dual role in both histone demethylation and protein ubiquitination.
• The protein localizes primarily to the nucleolus and chromosomes within the nucleus, consistent with its roles in regulating ribosomal RNA transcription and chromatin modification.
• Post-translational modifications include phosphorylation, and mutations in KDM2B have been associated with intellectual disability, highlighting its importance in normal development.
• Structurally, KDM2B contains several conserved domains: a CXXC zinc finger for DNA binding, an F-box for SCF complex interaction, leucine-rich repeats, and a JmjC domain responsible for its demethylase activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 600-700 of human KDM2B (XP_011537177.1). For Western blot, the expected molecular weight is ~153 kDa; consider using an appropriate gel percentage (e.g., 8% SDS-PAGE) and optimize transfer conditions for high molecular weight proteins.
• For ELISA applications, titrate the antibody using the immunogen peptide or recombinant protein as a coating antigen, and validate in a relevant sample system.
• As a rabbit polyclonal antibody, lot-to-lot consistency may vary; include appropriate positive and negative controls in each experiment and verify specificity in your target tissue or cell line.
• Cross-reactivity is specified for human; if using other species, validate due to sequence homology considerations, especially within the immunogen region.

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