Anti-KDM1/LSD1 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - O60341

Core Product Specifications and Parameters

Biological Background: KDM1/LSD1 Function and Localization

• KDM1/LSD1 (Lysine-specific histone demethylase 1A), also designated BHC110 or AOF2, is a FAD-dependent amine oxidase belonging to the flavin monoamine oxidase family.
• It specifically demethylates mono- and dimethylated lysine 4 (H3K4me1/2) and lysine 9 (H3K9me1/2) on histone H3, enabling it to act as a transcriptional coactivator or corepressor depending on the cellular context. Related references: PMID:15620353, PMID:15811342, PMID:16079794
• Efficient nucleosomal demethylation requires the corepressor RCOR1/CoREST, and LSD1-mediated H3K4 demethylation is essential for the repression of neuronal-specific genes. Related references: PMID:16079794, PMID:16140033, PMID:16885027
• In the presence of androgen receptor (AR), LSD1 switches to a coactivator role by demethylating H3K9me, a modification inhibited by PRKCB-mediated phosphorylation of histone H3 at Thr6. Related references: PMID:16079795
• LSD1 contributes to epithelial-to-mesenchymal transition (EMT) by acting as a downstream effector of SNAI1, repressing epithelial markers such as E-cadherin/CDH1. Related references: PMID:20562920, PMID:27292636
• Beyond histones, LSD1 demethylates non-histone substrates including DNMT1 and SOX2, modulating DNA methylation and stem cell pluripotency. Related references: PMID:29691401, PMID:29358331
• LSD1 is ubiquitously expressed, with notably high expression in brain and testis, where a neuron-specific isoform demethylates H4K20me1/2, a function essential for neuronal activity-dependent gene expression. Related references: PMID:26214369
• Subcellularly, LSD1 localizes predominantly in the nucleus associated with chromatin; it is annotated with keywords including chromatin regulator, transcription regulation, and developmental protein.

Experimental Guidance and Technical Tips

• The immunogen (aa 170–275) may be conserved across species, consistent with the reported cross-reactivity to human, mouse, rat, and monkey; verify target sequence homology in your model system.
• For Western blot, expect a band at approximately 93 kDa; consider nuclear extraction protocols to enrich the signal and use appropriate loading controls.
• In immunofluorescence and immunohistochemistry assays, optimize fixation and permeabilization conditions, as LSD1 is a nuclear protein; validate staining specificity with relevant controls.
• For ELISA, perform standard checkerboard titrations to determine optimal coating antigen and antibody concentrations for your specific plate format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material procurement, offering a comprehensive portfolio covering every stage from concept to clinic. This anti-KDM1/LSD1 antibody exemplifies our commitment to providing high-quality reagents for epigenetics and cancer research. In addition to this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to support your diagnostic development needs.