Anti-KDM1A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60341

Core Product Specifications and Parameters

Biological Background: Lysine-specific histone demethylase 1A Function and Localization

• Lysine-specific histone demethylase 1A (KDM1A), also known as LSD1, AOF2, BHC110, is a key histone demethylase that removes methyl groups from specific lysine residues on histones H3 and H4, thereby regulating gene expression.
• Demethylates mono- and di-methylated lysine 4 of histone H3 (H3K4me1/me2), generally associated with transcriptional activation, and dimethylated lysine 9 (H3K9me2), a repressive mark, acting as both a co-repressor and co-activator depending on the context. Related references: PMID:15620353, PMID:15811342, PMID:16079794, PMID:20389281, PMID:21300290
• Requires interaction with RCOR1/CoREST to demethylate nucleosomal H3K4me1/me2 and functions in complexes with HDACs to repress genes involved in blood cell development. Related references: PMID:16079794, PMID:16140033, PMID:16885027
• Predominantly located in the nucleus and associates with chromosomes, directly modifying histone methylation marks on chromatin.
• Ubiquitously expressed, with additional neuron-specific isoform(s) that demethylate H4K20me1/me2, crucial for spatial learning, memory, and neuronal gene regulation. Related references: PMID:26214369, PMID:25684206
• Required for gastrulation during embryogenesis; promotes epithelial-to-mesenchymal transition (EMT) by repressing epithelial markers like E-cadherin; implicated in Cushing syndrome and cancer. Related references: PMID:20562920, PMID:27292636, PMID:20389281
• Demethylates non-histone substrates including p53 (Lys370), DNMT1, and SOX2, thereby regulating DNA methylation and pluripotency. Related references: PMID:29691401, PMID:29358331
• Classified under keywords: Chromatin regulator, transcription co-regulator, FAD-dependent amine oxidase, phosphoprotein, developmental protein.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 130–380 within the amine oxidase domain; the polyclonal nature may yield robust signal across multiple applications, but batch-to-batch variability should be monitored.
• For Western blotting, expect a major band around 93 kDa; additional bands representing isoforms or post-translationally modified forms may appear. Validate detection with appropriate controls.
• In IHC-P and IF/ICC, optimize fixation and permeabilization to access the nuclear epitope; antigen retrieval may enhance signal. Since the antibody cross-reacts with mouse and rat, verify species-specific conditions.
• For ELISA, use the recombinant immunogen or purified protein as coating antigen to assess titer and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutions with a single-point access to high-quality IVD raw materials, trancing from concept to clinic. This anti-KDM1A antibody supports epigenetics and chromatin research, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.