Anti-KDM1 Rabbit Polyclonal Antibody for WB, IP, ELISA - O60341

Core Product Specifications and Parameters

Biological Background: KDM1 Function and Localization

• KDM1A encodes lysine-specific histone demethylase 1A (LSD1), a flavin-dependent amine oxidase that demethylates mono- and dimethylated histone H3 Lys-4 (H3K4me1/me2), acting as a transcriptional corepressor.
  Related references: PMID:15620353 PMID:15811342
• When complexed with RCOR1/CoREST, LSD1 mediates H3K4 demethylation on nucleosomes to repress neuronal gene expression.
  Related references: PMID:16079794 PMID:16140033
• In the context of androgen receptor signaling, LSD1 demethylates H3K9me1/me2 to function as a transcriptional coactivator; this specificity is modulated by histone H3 Thr-6 phosphorylation.
  Related references: PMID:16079794 PMID:16079795
• The enzyme also targets non-histone proteins, including demethylation of p53 at Lys-370 (impairing TP53BP1 binding), DNMT1 stabilization, and SOX2 regulation.
  Related references: PMID:29691401 PMID:29358331
• LSD1 is crucial for embryonic gastrulation and facilitates epithelial-mesenchymal transition (EMT) via SNAI1-mediated repression of E-cadherin and other epithelial markers.
  Related references: PMID:20562920 PMID:27292636
• In neurons, LSD1 demethylates histone H4 Lys-20 (H4K20me1/me2) to enable activity-dependent gene transcription, a process essential for spatial learning and memory.
  Related references: PMID:26214369 PMID:25684206
• The protein localizes to the nucleus and is associated with chromatin.
• Ubiquitously expressed, with prominent levels in brain and testis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to aa 365–515 within the catalytic amine oxidase domain, which is highly conserved across human, mouse, and rat, explaining the observed cross-reactivity.
• For Western blotting, a single band at approximately 93 kDa is expected; include appropriate controls for post-translational modifications that may shift molecular weight.
• In immunoprecipitation assays, use mild lysis conditions to preserve native protein complexes and consider co-immunoprecipitating known interactors like CoREST to confirm specificity.
• Although not listed as an application, this polyclonal antibody may also be suitable for chromatin immunoprecipitation (ChIP); however, validation under relevant chromatin conditions is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies procurement for diagnostic manufacturers and research teams by providing ready access to validated IVD raw materials like this anti-KDM1 (LSD1) antibody. Whether for epigenetic profiling or cancer biomarker development, we offer one-stop sourcing of polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized rare-target material services to streamline your workflow from early research to clinical application.