Anti-KCTD9 Monoclonal Antibody for WB, ELISA - Q7L273

Core Product Specifications and Parameters

Biological Background: KCTD9 Function and Localization

• KCTD9, also known as BTBD27, is a BTB/POZ domain-containing protein, characterized by the presence of a BTB (BR-C, ttk and bab) / POZ (Pox virus and Zinc finger) domain.
• The protein functions as a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their proteasomal degradation.
• KCTD9 is involved in the ubiquitin conjugation pathway, as indicated by keyword annotation (Ubl conjugation pathway).
• It is classified as a phosphoprotein, suggesting regulation by phosphorylation.
• The keyword "3D-structure" indicates that the three-dimensional structure of KCTD9 has been experimentally determined.
• The protein may contain repeated sequence motifs (keyword "Repeat").

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 1-100 of human KCTD9 (N-terminal region). For Western blot, validate antibody specificity using human cell lysates expressing endogenous KCTD9; the expected molecular weight is approximately 43 kDa.
• For ELISA applications, the antibody can be employed as a detection or capture reagent; consider pairing with a second antibody against a non-overlapping epitope to develop a sandwich assay.
• Since cross-reactivity is confirmed for human, appropriate human-derived samples should be used; for other species, verify cross-reactivity experimentally if required.
• Given that KCTD9 is a phosphoprotein, consider the potential impact of phosphorylation state on antibody recognition.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supplies high-quality anti-KCTD9 monoclonal antibodies and other reagents for ubiquitination research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development. Whether you need rare target sourcing or comprehensive technical support, CamelBio bridges your project from concept to clinic.