Anti-KCTD15 Polyclonal Antibody for WB and ELISA - Q96SI1

Core Product Specifications and Parameters

Biological Background: KCTD15 Function and Localization

• KCTD15, also known as potassium channel tetramerization domain-containing protein 15, is a member of the BTB/POZ domain-containing protein family.
• It contains a BTB/POZ domain, which mediates protein-protein interactions and is implicated in transcriptional regulation.
• During embryonic development, KCTD15 plays a critical role in neural crest formation (By similarity).
• KCTD15 inhibits AP2 transcriptional activity by interacting with its activation domain, thereby modulating gene expression (PubMed:23382213). Related references: PMID:23382213
• Localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• The gene undergoes alternative splicing, producing multiple isoforms; the canonical isoform has a molecular weight of approximately 32 kDa.
• KCTD15 is a phosphoprotein, and phosphorylation may regulate its activity and interactions.
• Mutations or variants in KCTD15 have been associated with disease phenotypes, highlighting its developmental importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-234 of human KCTD15, covering the full-length protein except possibly a short C-terminal region. This design may generate antibodies recognizing multiple isoforms.
• For Western blotting, a band of approximately 32 kDa is expected for the canonical isoform; additional bands from splice variants may be observed. Validate in the relevant sample system.
• For ELISA, consider coating with the immunogen or full-length recombinant protein as a positive control.
• Due to cross-reactivity with mouse and rat, this antibody is suitable for studies in rodent models; however, users should verify by sequence alignment and pilot experiments.
• Optimal working dilutions should be determined empirically for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between research and clinical supply by offering a comprehensive portfolio of IVD antibodies and ancillary reagents. This anti-KCTD15 polyclonal antibody exemplifies our commitment to providing high-quality raw materials for transcription and development studies. Contact us for your sourcing needs, from single batches to large-scale production.