Anti-KCNN3/SK3 Rabbit Monoclonal Antibody for WB, ELISA - Q9UGI6

Core Product Specifications and Parameters

Biological Background: KCNN3/SK3 Function and Localization

• The KCNN3 gene encodes two main protein isoforms: the full-length functional calcium-activated potassium channel and a shorter dominant-negative isoform that suppresses native SK channel activity by intracellular sequestration.
  Related references:
  PMID:12808432
• The full-length KCNN3 channel is gated by intracellular calcium bound to calmodulin, leading to voltage‑independent potassium efflux with a single‑channel conductance of ~10 pS and an inwardly rectifying current profile at positive membrane potentials.
  Related references:
  PMID:12808432, PMID:20562108, PMID:31155282
• KCNN3 contributes to neuronal excitability regulation by mediating the slow component of synaptic afterhyperpolarization (sAHP) following action potentials.
• KCNN3 is a member of the small conductance calcium‑activated potassium channel (SK) family, which includes KCNN1 (SK1) and KCNN2 (SK2).
• Subcellular localization includes the cell membrane, cytoplasm, and the Z line of sarcomeres in myofibrils.
• KCNN3 is widely expressed in human tissues, with relatively high abundance in the brain, representing 20–60% of total KCNN family transcripts in that organ.
• Post‑translational modifications include phosphorylation and constitutive calmodulin binding, which are essential for channel gating and regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 631–731, a region in the C‑terminal intracellular domain of human KCNN3; antibody specificity should be verified in the target sample.
• For Western blotting, a band around 81 kDa is expected; consider using brain lysate as a positive control given the higher expression in neural tissues.
• Cross‑reactivity with rat enables potential use in rodent models; validate in the specific tissue and experimental system.
• For ELISA applications, optimize the coating antigen and antibody dilution; sandwich ELISA may require paired antibodies.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is a one‑stop provider of IVD raw materials, offering validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents for diagnostic manufacturers and researchers. For potassium channel targets such as KCNN3, CamelBio supplies high‑quality antibodies and can assist with custom sourcing, technical consultation, and rare‑target raw‑material procurement to streamline your assay development.