Anti-KCNK9 Polyclonal Antibody for WB and ELISA - Q9NPC2

Core Product Specifications and Parameters

Biological Background: KCNK9 Function and Localization

• KCNK9 encodes the potassium channel subfamily K member 9, also known as TASK-3, TWIK-related acid-sensitive K+ channel 3, or KT3.2.
• Mediates voltage-dependent outward rectifying K+ currents via an ion flux gating mechanism; extracellular acidification protonates His-98, converting the channel to an Na+-permeable state (PubMed:11042359, PubMed:11431495, PubMed:26919430, PubMed:22948150, PubMed:38630723). This dual ion conductance contributes to neuronal and non-neuronal pH sensing. Related references: PMID:11042359 PMID:11431495 PMID:26919430 PMID:22948150 PMID:38630723
• Homo- and heterodimerizes with other KCNK subunits (e.g., KCNK3, KCNK1) to form functional channels with distinct regulatory properties (PubMed:23169818, PubMed:38630723). Heteromeric KCNK1:KCNK9 channels operate in hippocampal neurons, while KCNK3:KCNK9 complexes are found in cerebellar granule cells. Related references: PMID:23169818 PMID:38630723
• Subcellular localization includes the cell membrane, mitochondrial inner membrane, and dendrites, facilitating roles in both plasma membrane excitability and mitochondrial ion homeostasis.
• Predominantly expressed in the cerebellum, with additional presence in adrenal gland, kidney, and lung.
• In neurons, KCNK9 controls resting membrane potential and supports high-frequency action potential firing upon threshold; it is also implicated in retinal ganglion cell visual processing and respiratory chemoreflex modulation.
• Post-translational modifications include glycosylation (keyword), which may influence channel trafficking or stability.
• Associated with disease variants linked to intellectual disability (BIBARS syndrome), highlighting its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 265‑374) is located within a proposed intracellular loop of KCNK9. As a polyclonal antibody, it may detect multiple epitopes, including potential splice variants or post-translationally modified forms.
• For western blotting, consider using reducing conditions and validate the antibody with positive (e.g., cerebellum lysate) and negative control samples, given the reported cross-reactivity with human and mouse. Optimize primary antibody dilution in the range typically used for polyclonal antibodies.
• The predicted molecular weight is 42 kDa, but N-linked glycosylation may cause an upward shift; confirm the identity of the band by comparing with a molecular weight marker and, if available, a blocking peptide or knockdown sample.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable conduit to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For ion channel targets such as KCNK9, our network supports customized sourcing of polyclonal and monoclonal antibodies, enabling neuroscience and diagnostic assay development. Engage our team to streamline your reagent procurement and accelerate your project timeline.