Anti-KCNJ5 Polyclonal Antibody for WB, ELISA - P48544

Core Product Specifications and Parameters

Biological Background: KCNJ5 Function and Localization

• KCNJ5 encodes the G protein-activated inward rectifier potassium channel 4 (also known as GIRK4, Kir3.4, CIR, KATP-1), a member of the inwardly rectifying potassium channel family.
• It mediates potassium influx more readily than efflux, with voltage dependence modulated by extracellular potassium concentration; inward rectification primarily results from blockage of outward current by intracellular magnesium. The channel can be inhibited by external barium.
• Channel activity is controlled by G proteins, linking it to G protein-coupled receptor signaling pathways.
• The protein is localized to the plasma membrane (Membrane).
• Expressed in pancreatic islets, exocrine pancreas, heart, and adrenal cortex (particularly zona glomerulosa), consistent with its roles in hormone secretion and cardiac excitability.
• Classified as a phosphoprotein, suggesting regulation by phosphorylation.
• Mutations in KCNJ5 are associated with long QT syndrome (LQT13) and other cardiac disorders (Disease variant).

Experimental Guidance and Technical Tips

• For Western blot, consider using membrane protein extraction protocols to enrich KCNJ5, which may improve detection of the ~48 kDa band.
• Peptide blocking assays or knockout/knockdown validation are recommended to confirm antibody specificity.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for rodent model studies; adjust sample preparation and detection conditions accordingly.
• For ELISA applications, the immunogen peptide may serve as a positive control or coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibodies, antibody pairs, and bulk ancillary reagents for potassium channel targets like KCNJ5. Our portfolio supports cardiovascular and endocrine assay development, and we offer rare target sourcing and technical consulting from concept to clinic. Contact us for reliable, scale-ready supply solutions.