Anti-KCNJ4 Polyclonal Antibody for WB, IHC-P, ELISA - P48050

Core Product Specifications and Parameters

Biological Background: Inward Rectifier Potassium Channel 4 (KCNJ4) Function and Localization

• Encodes the inward rectifier potassium channel 4 (KCNJ4), also known as HIRK2, HRK1, Kir2.3, IRK-3, and hippocampal inward rectifier. It belongs to the inwardly rectifying potassium channel subfamily J.
• Mediates potassium influx with a strong inward rectification property: potassium flows into the cell more readily than out, governed by extracellular potassium levels and blocked by intracellular magnesium.
• The channel can be blocked by extracellular barium and cesium ions, a hallmark of inward rectifier channels.
• Localizes to the cell membrane, postsynaptic cell membrane, and cytoplasmic vesicle membrane, consistent with roles in membrane excitability and signaling.
• Predominantly expressed in heart, skeletal muscle, and specific brain regions including the hippocampus.
• Key structural features: multiple transmembrane helices, voltage-gated channel domain, and involvement in potassium transport.
• The UniProt-reported molecular weight is approximately 49.5 kDa, consistent with the observed 50 kDa band in experimental systems.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–150 of human KCNJ4 (NP_004972.1). This region corresponds to an intracellular loop; consider verifying sequence conservation across species and isoforms to predict cross-reactivity.
• For Western blot, a band near 50 kDa is expected in lysates from heart, skeletal muscle, or brain tissue (human, mouse, rat). Use appropriate positive and negative controls.
• In immunohistochemistry (paraffin), optimize antigen retrieval and antibody dilution using tissue sections known to express KCNJ4 (e.g., hippocampus or heart).
• For ELISA, the peptide immunogen can serve as a positive control. Validate coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with convenient access to validated IVD raw materials, including optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This Anti-KCNJ4 antibody supports potassium channel and ion transport studies, and we can also assist with matched antibody pairs or custom reagent sourcing to accelerate your diagnostic or research workflow. With end-to-end support from concept to clinic, CamelBio is your reliable partner for critical raw-material procurement.