Anti-KCNJ2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P63252

Core Product Specifications and Parameters

Biological Background: KCNJ2 (Kir2.1) Function and Localization

• KCNJ2 encodes the inward rectifier potassium channel Kir2.1, which permits potassium influx more readily than efflux, a property essential for maintaining resting membrane potential and controlling excitability in excitable cells. Related references: PMID:36149965, PMID:7590287, PMID:9490857
• The voltage dependence of Kir2.1 is modulated by extracellular potassium concentration; elevated external potassium shifts the activation range to more positive voltages. Related references: PMID:7590287, PMID:7696590
• Outward current through the channel is blocked by intracellular magnesium, and the channel can be inhibited by extracellular barium or cesium ions. Related references: PMID:9490857, PMID:7590287, PMID:7696590
• Kir2.1 contributes to the establishment of the action potential waveform and regulates excitability in neuronal and muscle tissues. Related references: PMID:7590287, PMID:7696590, PMID:7840300
• The protein localizes to the cell membrane, specifically enriched in the sarcolemma and T-tubules of muscle cells.
• KCNJ2 is expressed in heart, brain, placenta, lung, skeletal muscle, and kidney, with diffuse distribution throughout the brain.
• Post-translational modifications include S-nitrosylation and disulfide bond formation; mutations in KCNJ2 are associated with atrial fibrillation, long QT syndrome type 7 (LQT7), and short QT syndrome type 3 (SQT3).

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal intracellular domain (amino acids 318-427); the antibody is expected to detect total KCNJ2 protein in relevant sample types.
• For western blot, an approximate band at 48kDa is anticipated; validate molecular weight and specificity in your specific lysate and experimental conditions.
• For IHC-P, anticipate membrane-localized staining; optimize antigen retrieval and antibody dilution for formalin-fixed paraffin-embedded tissue sections.
• For ELISA, the recombinant protein immunogen can serve as a positive control to evaluate antibody titer and binding characteristics.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For cardiac ion channel and neuroscience research, the anti-KCNJ2 antibody supports assay development, while our team can assist with sourcing rare target reagents to accelerate your diagnostic projects.