Anti-KCNJ15 Polyclonal Antibody for WB, IHC-P, ELISA - Q99712

Core Product Specifications and Parameters

Biological Background: KCNJ15 Function and Localization

• KCNJ15 encodes ATP-sensitive inward rectifier potassium channel 15, also known as Kir1.3 or Kir4.2, a member of the inwardly rectifying potassium (Kir) channel family.
• Inward rectifier potassium channels exhibit a greater tendency to allow potassium flow into the cell; their voltage dependence is modulated by extracellular K+ concentration, shifting activation to more positive voltages as external potassium rises.
• The inward rectification is primarily due to blockage of outward potassium current by intracellular magnesium ions, ensuring unidirectional ion flux.
• KCNJ15 localizes to the cell membrane, positioning it to regulate membrane potential and potassium homeostasis.
• According to UniProt, KCNJ15 is classified under keywords including 'Voltage-gated channel', 'Ion transport', 'Potassium transport', and 'Proteomics identification', highlighting its functional role and experimental evidence.
• The protein has a molecular weight of approximately 43 kDa (product specifications) and 42577 Da (UniProt); minor variations may arise from post-translational modifications or experimental conditions.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against a recombinant fragment corresponding to the C-terminal region (aa 221-375), it may recognize multiple epitopes, potentially reducing loss of signal due to minor antigen variations.
• For Western blotting, the target protein has a predicted molecular weight of ~43 kDa; include appropriate molecular weight markers and load positive control lysates from KCNJ15-expressing tissues/cell lines (e.g., renal or pancreatic) to confirm specificity.
• In IHC-P, optimize antigen retrieval conditions and validate staining in tissues known to express KCNJ15, such as kidney or pancreas; consider using peptide blocking to verify signal.
• For ELISA, the antibody may be used as a capture or detection reagent depending on assay design; titration is recommended to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. For studies targeting ion channels such as KCNJ15, we provide reliable raw materials and sourcing services to streamline your assay development and production workflows. Leverage our one-stop access to accelerate your project from concept to clinic.