Anti-KCNJ12 Rabbit Polyclonal Antibody for WB, ELISA - Q14500

Core Product Specifications and Parameters

Biological Background: KCNJ12 Function and Localization

• KCNJ12 encodes the ATP-sensitive inward rectifier potassium channel 12 (Kir2.2), also known as IRK-2, inward rectifier K(+) channel Kir2.2, or hIRK1.
• It belongs to the inwardly rectifying subfamily J of potassium channels and plays a critical role in controlling resting membrane potential and action potential waveform in electrically excitable cells such as neurons and muscle.
• Inward rectifier potassium channels exhibit greater potassium influx than efflux; their voltage dependence is modulated by extracellular potassium concentration, where higher external K+ shifts channel opening to more positive voltages.
• The inward rectification mechanism is primarily attributed to blockage of outward potassium current by intracellular magnesium.
• The channel is localized to the cell membrane, specifically the sarcolemma and T-tubules in muscle cells.
• Post-translational modifications include S-nitrosylation, disulfide bond formation, and metal-binding, which may regulate channel function.
• Keywords: Cell membrane, Ion channel, Potassium transport, Voltage-gated channel, Transmembrane helix, S-nitrosylation, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to the C-terminal region (aa 354-433) of human KCNJ12; consider that this polyclonal antibody may recognize multiple epitopes within this region.
• For western blot (WB), the predicted molecular weight is ~49 kDa; however, possible post-translational modifications or splice variants may result in additional bands—validate with appropriate controls.
• Given the reported cross-reactivity with mouse and rat, this antibody can be a useful tool for comparative studies across species, but confirm reactivity in your specific experimental system.
• For ELISA applications, optimize coating conditions and antibody dilution; polyclonal antibodies may exhibit lot-to-lot variation, so include internal references when switching lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials and technical support, streamlining your procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For research on potassium channels and excitable cell physiology, this anti-KCNJ12 antibody fits into a broader portfolio that can be tailored to your assay development and diagnostic manufacturing needs. From rare target sourcing to ready-to-use raw materials, CamelBio bridges the gap between concept and clinic.