Anti-KCNH6 Polyclonal Antibody for WB, ELISA - Q9H252

Core Product Specifications and Parameters

Biological Background: KCNH6 Function and Localization

• KCNH6 encodes the pore-forming alpha subunit of a voltage-gated inwardly rectifying potassium channel, also known as Kv11.2, hERG2, or ERG-2.
• The channel exhibits unusual gating kinetics: it produces relatively small outward currents during membrane depolarization and large inward currents during repolarization, due to rapid inactivation and slow deactivation.
• It activates even more slowly than the related KCNH2 (hERG1) channel, contributing to distinct biophysical properties.
• KCNH6 is localized to the cell membrane and is predicted to be a glycoprotein with multiple transmembrane domains.
• The gene undergoes alternative splicing, generating protein isoforms; the full-length canonical isoform has a molecular weight of approximately 106 kDa (110 kDa as detected in western blot potentially due to glycosylation).
• Tissue expression is notably observed in prolactin-secreting adenomas, suggesting a role in pituitary cell excitability or hormone secretion.
• As a member of the ether-a-go-go-related (ERG) potassium channel subfamily, KCNH6 is involved in potassium ion transport and membrane potential regulation.

Experimental Guidance and Technical Tips

• The immunogen (aa 70–220) targets an intracellular N-terminal region, which is likely well-exposed; consider using a blocking peptide or knockdown control to confirm specificity.
• For Western blot, expect a band near 110 kDa; additional bands may represent glycosylated forms or alternative splice variants.
• In ELISA, the antibody can be used as a capture or detection reagent; optimize coating and detection conditions based on your plate format and substrate.
• Validate the antibody in human cell lines or tissues known to express KCNH6, such as prolactin-secreting adenoma samples or transfected cells.
• Since it is a polyclonal preparation, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. For potassium channel targets like KCNH6, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Let CamelBio be your sourcing bridge to reliable, high-quality raw materials for even the most challenging protein targets.