Anti-KCNH2 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q12809

Core Product Specifications and Parameters

Biological Background: KCNH2 Function and Localization

• KCNH2 (HERG/Kv11.1) is the pore-forming alpha subunit of a voltage-gated inwardly rectifying potassium channel characterized by slow deactivation and rapid inactivation kinetics, producing small outward currents during depolarization and large inward currents upon repolarization. Related references: PMID:10219239 PMID:10753933 PMID:10790218 PMID:10837251
• It forms a stable heteromeric complex with KCNE1 or KCNE2, which regulates its inward rectifier potassium channel activity. Related references: PMID:10219239 PMID:9230439
• Some KCNH2 isoforms lack intrinsic channel activity but modulate channel characteristics by forming heterotetramers, and these isoforms are retained intracellularly and degraded via the ubiquitin pathway.
• The channel localizes to the cell membrane.
• KCNH2 is highly expressed in the heart and brain, playing a critical role in cardiac repolarization and neuronal excitability. Isoforms such as USO are frequently overexpressed in cancer cells.
• Post-translational modifications include glycosylation, phosphorylation, and methylation, and mutations in KCNH2 are associated with long QT syndrome and short QT syndrome.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide corresponding to amino acids 850-950 of human KCNH2 (NP_000229.1). It is expected to recognize both native and denatured forms of the protein.
• For Western blotting, the predicted molecular weight is approximately 127 kDa. Use appropriate loading controls and validate in your sample system.
• For immunofluorescence and immunohistochemistry, consider using heart or brain tissue sections as positive controls, given the high expression in these tissues.
• The antibody cross-reacts with human and mouse, but performance in other species should be verified.
• As with any polyclonal antibody, lot-to-lot variability may occur; it is recommended to titrate the antibody for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-KCNH2 polyclonal antibody supports research into cardiac ion channel function, neurological disorders, and cancer biology. Leverage our supply network for validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare targets to accelerate your assay development from concept to clinic.