Anti-KCNH1 Polyclonal Antibody for WB, ELISA - O95259

Core Product Specifications and Parameters

Biological Background: KCNH1 Function and Localization

• KCNH1 encodes the pore-forming alpha subunit of a voltage-gated delayed rectifier potassium channel that mediates outward-rectifying potassium currents which do not inactivate upon depolarization (PubMed:10880439, PubMed:22732247).
  Related references: PMID:10880439 PMID:22732247
• Channel activation kinetics are dependent on prepulse potential and external divalent cation concentration, exhibiting a biphasic time course with fast and slowly activating components (PubMed:11943152).
  Related references: PMID:11943152
• Channel properties are modulated by subunit assembly (PubMed:11943152).
  Related references: PMID:11943152
• KCNH1 mediates IK(NI) current in myoblasts (PubMed:9738473).
  Related references: PMID:9738473
• Involved in the regulation of cell proliferation and differentiation, particularly adipogenic and osteogenic differentiation in bone marrow-derived mesenchymal stem cells (MSCs) (PubMed:23881642).
  Related references: PMID:23881642
• KCNH1 localizes to the cell membrane, nuclear inner membrane, dendrites, axons, presynaptic and postsynaptic membranes, perikaryon, and early endosome membrane.
• Highly expressed in brain and in myoblasts at the onset of fusion, with detection in certain cancer cell lines (e.g., HeLa, SH-SY5Y, MCF-7) but not in normal epithelial cells.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 813-962, a C-terminal region of human KCNH1. This polyclonal antibody may recognize the full-length protein (~111 kDa) in Western blot assays; consider using reducing SDS-PAGE conditions.
• For ELISA applications, validate the antibody against the recombinant immunogen or full-length protein under your experimental conditions.
• Given the reported cross-reactivity with human, mouse, and rat, this antibody can be evaluated in rodent models. Test in relevant tissues such as brain or myoblast cell lines.
• KCNH1 exhibits complex subcellular localization; fixation and permeabilization protocols should be optimized if using this antibody for immunostaining.
• Include appropriate positive and negative controls (e.g., brain lysate, myoblast lysate, KCNH1 knockout samples) to confirm specificity.

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