Anti-KCNE1 Rabbit Polyclonal Antibody for WB, ELISA - P15382

Core Product Specifications and Parameters

Biological Background: KCNE1 Function and Localization

• Potassium voltage-gated channel subfamily E member 1 (KCNE1), also known as MinK, ISK, JLNS, or LQT5, is a single-pass transmembrane protein that functions as a regulatory beta subunit of voltage-gated potassium (Kv) channels.
• It associates with KCNQ1/KVLQT1 alpha subunit to form the slowly activating delayed rectifier cardiac potassium (IKs) channel, which is critical for ventricular muscle action potential repolarization.
  Related references:
  PMID:20533308
• KCNE1 modulates the gating kinetics of the delayed rectifier Kv channel containing KCNB1 alpha subunit, altering its functional properties.
  Related references:
  PMID:19219384
• Assembly with KCNH2/HERG alpha subunit may regulate the rapidly activating component (IKr) of the delayed rectifying potassium current in the heart.
  Related references:
  PMID:9230439
• The protein is localized to the cell membrane, enriched in the apical membrane of epithelial cells and membrane rafts.
• Tissue expression includes heart, lung, kidney, testis, ovaries, small intestine, and peripheral blood leukocytes; it is absent in pancreas, spleen, prostate, and colon.
• Post-translational modifications include N-linked glycosylation and phosphorylation, which may regulate channel function.
• Mutations in KCNE1 are associated with Long QT syndrome type 5 (LQT5) and the autosomal recessive Jervell and Lange-Nielsen syndrome (JLNS), characterized by cardiac arrhythmias and congenital deafness.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (amino acids 1-100) of human KCNE1, suitable for detecting both human and mouse proteins with predicted molecular weight ~15 kDa.
• For Western blot, consider enriching membrane fractions to improve detection of this transmembrane protein.
• Validate antibody specificity in your experimental system using appropriate controls, such as overexpression lysates or peptide competition assays.
• The antibody is validated for ELISA; optimize coating conditions and detection parameters for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop platform supplying IVD raw materials, technical services, and consulting to drive diagnostic development from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents—ideal for cardiac ion channel research on targets like KCNE1. Leverage our rare target sourcing capabilities and expert support to accelerate your assay development.