Anti-KCND3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UK17

Core Product Specifications and Parameters

Biological Background: KCND3 Function and Localization

• KCND3 encodes the pore-forming alpha subunit of voltage-gated A-type potassium channels, known as Kv4.3, responsible for rapidly activating and inactivating potassium currents in excitable cells. Related references: PMID:10200233 PMID:17187064 PMID:21349352
• In cardiomyocytes, KCND3 contributes to the transient outward potassium current (Ito) essential for cardiac action potential repolarization; in neurons, it mediates somatodendritic A-type currents (ISA) regulating synaptic integration and firing frequency. (By similarity)
• Channel gating kinetics—activation, inactivation, and recovery—are modulated by auxiliary subunits such as KCNIP1, KCNIP2, and DPP6, forming macromolecular complexes that fine-tune electrical signaling. Related references: PMID:17187064 PMID:34997220
• KCND3 is primarily localized at the cell membrane, including the sarcolemma in cardiac muscle and dendrites in neurons, consistent with its role in surface electrical excitability.
• Tissue distribution shows high expression in the brain (cortex, cerebellum, amygdala, caudate nucleus) and heart, with lower levels detected in liver, skeletal muscle, kidney, and pancreas. Related references: PMID:10200233 PMID:10729221 PMID:9843794
• Mutations in KCND3 are linked to human diseases including Brugada syndrome (cardiac arrhythmia) and spinocerebellar ataxia types 19 and 22 (neurodegeneration), underscoring its critical role in excitable tissues.
• The protein undergoes alternative splicing, yielding isoforms with distinct properties, and contains multiple phosphorylation sites and a zinc-binding domain that may regulate channel function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 521–655 of human KCND3, a C-terminal region; polyclonal antibodies raised against this fragment likely recognize endogenous full-length protein and possibly some isoforms.
• Western blot (WB) analysis predicts a band around 73 kDa; validate specificity using appropriate positive controls such as human heart or brain lysates, and consider confirming with knockdown or blocking peptide experiments.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody concentration should be optimized; expected membrane and dendritic staining may be observed in tissue sections.
• ELISA applications may require coated recombinant protein or peptides; assess reactivity against the immunogen and cross-reactive species (mouse, rat) based on sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting for diagnostic development. Our catalog includes validated antibody pairs, optimized polyclonal antibodies like this anti-KCND3 rabbit pAb, and bulk ancillary reagents to support neuroscience and cardiac biomarker research. Whether you need custom antibody generation or rare target sourcing, CamelBio streamlines your transition from concept to clinical application.