Anti-KCNAB3 Rabbit Polyclonal Antibody for WB and ELISA - O43448

Core Product Specifications and Parameters

Biological Background: KCNAB3 Function and Localization

• KCNAB3 encodes voltage-gated potassium channel subunit beta-3 (Kv-beta-3), a regulatory beta subunit of voltage-gated potassium (Kv) channels that modulates pore-forming alpha subunit activity. Related references: PMID:9857044
• The beta-3 subunit increases inactivation of Kv1.5/KCNA5 alpha subunit-containing channels, as demonstrated in functional studies. Related references: PMID:9857044
• It may mediate channel closure (By similarity) and exhibits potential nicotinamide adenine dinucleotide phosphate (NADPH)-dependent aldoketoreductase activity, with NADP(H) cofactor binding implicated in channel regulation (By similarity).
• KCNAB3 is localized to the cytoplasm.
• Expression is brain-specific, with highest levels in the cerebellum and weaker signals in the cortex, occipital lobe, frontal lobe, and temporal lobe. It is absent from spinal cord, heart, lung, liver, kidney, pancreas, placenta, and skeletal muscle.
• Associated keywords include cytoplasm, ion channel, ion transport, NADP, oxidoreductase, potassium, potassium transport, and voltage-gated channel, reflecting its dual enzymatic and channel regulatory roles.

Experimental Guidance and Technical Tips

• For Western blot analysis, the predicted molecular weight of KCNAB3 is approximately 44 kDa; use appropriate gel percentage and transfer conditions.
• Given its brain-specific expression, consider using cerebellum or cerebral cortex lysates as positive controls.
• The antibody's verified cross-reactivity with mouse enables its use in mouse brain tissue studies, but validate in your specific model system.
• For ELISA, titrate the antibody to determine optimal coating and detection conditions for your assay format.
• As a polyclonal antibody, it may recognize multiple epitopes, potentially increasing sensitivity but necessitating careful blocking and washing steps to reduce background.

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