Anti-KCNAB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13303

Core Product Specifications and Parameters

Biological Background: KCNAB2 Function and Localization

• KCNAB2 encodes the voltage-gated potassium channel subunit beta-2 (Kv-beta-2), a regulatory subunit of Shaker-type voltage-gated potassium (Kv) channels composed of pore-forming alpha subunits and regulatory beta subunits.
• The beta-2 subunit promotes potassium channel closure and inactivation of specific alpha subunit-containing channels, including Kv1.4/KCNA4 and Kv1.5/KCNA5, thereby modulating neuronal excitability and signal transduction (PubMed:11825900, PubMed:7649300). Related references: PMID:11825900 PMID:7649300
• It also exhibits NADPH-dependent aldoketoreductase activity, catalyzing the reduction of a broad range of aldehyde and ketone substrates (though no physiological substrate has been identified).
• Subcellularly, KCNAB2 localizes to the cytoplasm, cell membrane, neuronal axons, synapses, and synaptosomes, and associates with the cytoskeleton, consistent with its role in ion channel regulation at specialized membrane domains.
• Tissue expression includes myelinated nerve fibers in the spinal cord, juxtaparanodal and paranodal regions of nodes of Ranvier, and the hippocampus, suggesting involvement in central and peripheral nerve conduction.
• Post-translational modifications include acetylation and phosphorylation, and the protein functions in an NADP-dependent manner; alternative splicing generates multiple isoforms.
• KCNAB2 is classified under keywords such as ion transport, potassium transport, oxidoreductase, and synapse, highlighting its dual enzymatic and channel-regulatory roles.
• Gene ID in human is 8514; the protein molecular weight is approximately 41 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 40 amino acids of human KCNAB2 (NP_003627.1), which may allow detection of isoforms that include this region. Verify specificity in your experimental system, especially if alternative splicing isoforms are present.
• For Western blot (WB), the expected molecular weight is approximately 41 kDa for the full-length protein; use appropriate positive controls (e.g., human brain lysates) and validate with siRNA knockdown if possible.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider that KCNAB2 localizes to the cytoplasm, membrane, and axons; permeabilization and fixation conditions should be optimized, and co-staining with axonal or synaptic markers is recommended.
• For ELISA, titrate the antibody using recombinant KCNAB2 protein or peptide to determine optimal coating and detection concentrations, and include a blocking step to reduce non-specific binding.
• The antibody is predicted to cross-react with mouse and rat KCNAB2 based on sequence homology; however, confirm cross-reactivity experimentally by testing in relevant rodent tissues.
• As a polyclonal antibody, lot-to-lot variability may occur; consider validating each new lot and storing aliquots at -20°C to -80°C as recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents tailored to your immunoassay development. For neuroscience and ion channel research, we provide ready-to-integrate KCNAB2 antibodies and can source rare reagents to accelerate your diagnostic projects from concept to clinic.