Anti-KCNA4 Polyclonal Antibody for WB and ELISA - P22459

Core Product Specifications and Parameters

Biological Background: KCNA4 Function and Localization

• KCNA4, also known as Kv1.4, HK1, HPCN2, and other aliases, is a voltage-gated potassium channel subunit that mediates transmembrane potassium transport in excitable membranes. Related references: PMID:19912772, PMID:8495559
• The channel forms tetrameric complexes and can exist as functional homotetrameric channels or heterotetrameric channels with other KCNA subunits such as KCNA1, KCNA2, and KCNA5, where the subunit composition influences channel properties. Related references: PMID:8495559
• Homotetrameric KCNA4 channels open in response to membrane depolarization and then undergo rapid spontaneous inactivation. Related references: PMID:19912772, PMID:8495559
• Heterotetrameric channels containing KCNA1 and KCNA4 also exhibit rapid inactivation kinetics. Related references: PMID:17156368
• KCNA4 is located primarily at the cell membrane and is enriched in axons.
• Tissue expression includes brain, with lower levels in testis, lung, kidney, colon, and heart; detected in heart ventricle. Related references: PMID:27582084
• Post-translational modifications include N-glycosylation and phosphorylation, contributing to channel regulation; disease variants are linked to cataract, dystonia, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (AA 250–350) of human KCNA4. For Western blot, consider using membrane-enriched protein extracts from brain or cardiac tissue to enhance detection.
• The predicted molecular weight is approximately 73 kDa. Due to potential glycosylation and other modifications, the observed band may appear slightly higher; validate with appropriate controls.
• For ELISA, direct coating with the immunogen peptide can serve as a positive antigen control. Confirm specificity through pre-adsorption experiments.
• Cross-reactivity with mouse and rat has been reported; however, validation in the target species and tissue system is recommended.
• As a polyclonal antibody, batch-to-batch consistency should be verified, and inclusion of blocking peptides or knockout lysates is advised for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and laboratories with reliable access to high-quality research reagents. This anti-KCNA4 polyclonal antibody is well-suited for studies on voltage-gated potassium channels in neuroscience and cardiovascular research. Through our global network, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare-target raw materials to accelerate your assay development and research workflows.