Anti-KCNA2 Polyclonal Antibody for WB, IF/ICC, ELISA - P16389

Core Product Specifications and Parameters

Biological Background: KCNA2 Function and Localization

• KCNA2 (Kv1.2) is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and CNS. It forms tetrameric potassium-selective channels that open and close in response to membrane voltage changes (PubMed:11211111, PubMed:19912772, PubMed:23769686). Related references: PMID:11211111, PMID:19912772, PMID:23769686
• Homotetrameric KCNA2 channels function as delayed-rectifier potassium channels that open upon membrane depolarization, followed by slow spontaneous closure, while heterotetramers with KCNA4 exhibit rapid inactivation (PubMed:8495559, PubMed:19912772, PubMed:23769686). Related references: PMID:8495559, PMID:19912772, PMID:23769686
• KCNA2 plays a critical role in regulating neuronal excitability and preventing aberrant action potential firing. It acts presynaptically to suppress dendritic hyperexcitability and contributes to motor coordination (By similarity).
• Subcellularly, KCNA2 localizes to the cell membrane, axon, dendrites, synapse, presynaptic membrane, and endoplasmic reticulum membrane, reflecting its dynamic role in neuronal signaling.
• Channel properties are modulated by cytoplasmic beta subunits, which regulate subcellular localization and promote rapid inactivation of delayed rectifier potassium currents (PubMed:20220134). Related references: PMID:20220134
• KCNA2 is detected in brain cortex, peroneal nerve juxtaparanodal regions of the node of Ranvier, and paranodal/juxtanodal zones in myelinated spinal cord. Reduced expression is linked to diabetic axonal neuropathy (PubMed:16473933, PubMed:22649228, PubMed:11086297). Related references: PMID:16473933, PMID:22649228, PMID:11086297
• KCNA2 dysfunction is associated with neurological disorders including epileptic encephalopathy type 32 (DEE32), autism spectrum disorder, and intellectual disability, underscoring its importance in brain function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–165) of human KCNA2, which is highly conserved. This may allow recognition of full-length and certain truncated isoforms; validate in your sample system.
• For Western blot, a band at ~57 kDa is expected, corresponding to the calculated molecular weight. Additional bands may represent glycosylated or complexed forms.
• In IF/ICC, consider optimizing permeabilization and fixation conditions to detect membrane-localized Kv1.2 channels. Co-staining with synaptic markers may help confirm subcellular localization.
• This antibody is suitable for ELISA; ensure proper coating of recombinant or native antigen for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of validated antibodies, bulk reagents, and sourcing solutions. Our KCNA2 polyclonal antibody joins a growing catalog for ion channel and neuroscience research, backed by technical consultation to streamline your development pipeline. From rare target procurement to ancillary reagent supply, CamelBio bridges the gap from concept to clinic.