Anti-KCNA1 Polyclonal Antibody for WB, IF-P, ELISA - Q09470

Core Product Specifications and Parameters

Biological Background: KCNA1 Function and Localization

• KCNA1 encodes the pore-forming alpha subunit of a voltage-gated delayed-rectifier potassium channel (Kv1.1), mediating transmembrane K+ transport in excitable membranes, primarily in brain and kidney. Related references: PMID:19903818 PMID:8845167
• The channel forms homo- and heterotetramers with other KCNA family members (KCNA2, KCNA4, etc.); subunit composition determines channel kinetics, ranging from slow-inactivating (homotetrameric) to rapidly inactivating (heteromeric with KCNA4). Related references: PMID:12077175 PMID:17156368 PMID:19903818 PMID:19912772
• KCNA1 is critical for regulating membrane potential and preventing neuronal hyperexcitability, particularly in the hippocampus, cerebellum, and vagus nerve, influencing action potential firing, neurotransmitter release (e.g., GABA, dopamine), and heart contraction. Related references: PMID:17156368 PMID:11026449 PMID:17136396
• Expressed at the cell membrane and along axons, dendrites, and presynaptic terminals; also found in cytoplasmic vesicles, endoplasmic reticulum, and at paranodal/juxtaparanodal regions of myelinated axons. Related references: PMID:11086297
• Post-translationally modified by palmitoylation, phosphorylation, and N-glycosylation; classified as a lipoprotein, phosphoprotein, and glycoprotein, with voltage-sensing transmembrane helices.
• Plays a role in renal magnesium reabsorption in the distal convoluted tubule, contributing to Mg2+ homeostasis. Related references: PMID:19307729 PMID:23903368
• Detected at the protein level in spinal cord nerve fibers and islets of Langerhans; mutations in KCNA1 are associated with episodic ataxia and myokymia syndromes.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 426-495) lies within the intracellular C-terminal domain, suggesting the antibody recognizes an epitope accessible in permeabilized samples; consider permeabilization for IF-P.
• For Western blot, the predicted molecular weight is 56 kDa, but glycosylation may cause slight band shifts; validate in lysates from brain or kidney tissues.
• KCNA1 expression is enriched in neuronal tissues; use positive controls such as human, mouse, or rat brain lysates to confirm specificity.
• In ELISA, pair with a carefully validated detection antibody to avoid cross-reactivity with other Kv1 family members due to high sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for diagnostic manufacturers and research laboratories, offering validated IVD raw materials like anti-KCNA1 antibodies for studies on neuronal excitability, channelopathies, and renal magnesium transport. Our portfolio includes optimized polyclonal and monoclonal antibodies, matched antibody pairs for assay development, and bulk ancillary reagents, along with technical consulting to support your project from concept to clinical application.