Anti-KBTBD8 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NFY9

Core Product Specifications and Parameters

Biological Background: KBTBD8 Function and Localization

• KBTBD8 (Kelch repeat and BTB domain-containing protein 8), also known as T-cell activation kelch repeat protein (TA-KRP), is a member of the Kelch-like family of BTB domain-containing proteins.
• It functions as a substrate-specific adapter for the BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex, mediating monoubiquitination of target proteins such as NOLC1 and TCOF1. Related references: PMID:26399832
• Monoubiquitination by the BCR(KBTBD8) complex promotes formation of a NOLC1-TCOF1 complex that connects RNA polymerase I with ribosomal processing machinery, remodeling the translational program to favor neural crest specification during development.
• Subcellular localization studies indicate that KBTBD8 localizes to the cytoplasm, and is associated with the cytoskeleton, mitotic spindle, and Golgi apparatus.
• The protein is involved in translation regulation and the ubiquitin conjugation pathway, as reflected by its UniProt keywords.
• KBTBD8 contains Kelch repeats and a BTB domain, characteristic of substrate adapters in Cullin-RING E3 ligase complexes.
• Alternative splicing of the KBTBD8 gene generates multiple transcript variants, potentially giving rise to isoforms with distinct functions or localizations.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 50–280 of human KBTBD8, encompassing the BTB domain and part of the kelch repeat region, which may provide broad epitope coverage.
• For Western blotting, a band around 69 kDa is expected (UniProt calculated mass: 68.8 kDa). Optimize blocking and antibody dilution using the relevant sample lysate.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider permeabilization protocols compatible with the protein's reported cytoplasmic and organellar localization (spindle, Golgi).
• Cross‑reactivity with mouse and rat KBTBD8 makes this antibody suitable for rodent model studies; validation in the specific experimental system is recommended.
• Include appropriate positive and negative controls, such as lysates from KBTBD8‑overexpressing cells or knockout lines, to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw‑material procurement, from discovery to commercialization. This KBTBD8 polyclonal antibody is part of our extensive catalog of validated antibodies for ubiquitin proteasome pathway and developmental biology targets. We offer bulk supply, custom polyclonal and monoclonal antibody development, and sourcing for rare research reagents to streamline your diagnostic or research workflows.