Anti-KATNB1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BVA0

Core Product Specifications and Parameters

Biological Background: KATNB1 Function and Localization

• KATNB1 encodes the p80 regulatory subunit of the katanin complex, an AAA ATPase that severs microtubules in an ATP-dependent manner.
• The p80 subunit targets the enzymatic p60 subunit to specific subcellular sites, including the centrosome and mitotic spindle poles, enabling localized microtubule severing.
• Microtubule severing by katanin promotes rapid reorganization of the cytoskeleton, facilitates microtubule release from nucleation sites, and contributes to poleward microtubule flux and chromosome movement during mitosis.
• In neurons, KATNB1-mediated microtubule release is required for transport of microtubules into developing processes, which is essential for axonal growth.
• Subcellular localization is primarily cytoplasmic, with enrichment at the centrosome, spindle poles, and throughout the cytoskeleton.
• Mutations in KATNB1 are associated with lissencephaly (LIS6), a brain malformation disorder, highlighting its critical role in cortical development.
• The protein features multiple WD40 repeat domains, likely mediating protein-protein interactions within the katanin complex and with other regulators.
• KATNB1 is subject to phosphorylation, which may modulate its activity; its apparent molecular weight is approximately 72 kDa.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human, mouse, or rat cell lines; expect a major band near 72 kDa corresponding to full-length KATNB1.
• In IF/ICC, fixation and permeabilization protocols should be optimized to preserve centrosomal and spindle pole staining; co-staining with a centrosome marker (e.g., γ-tubulin) is recommended for validation.
• The immunogen sequence (aa 400–500) lies in the C-terminal region, so the antibody may recognize both full-length protein and potential splice variants or degradation products.
• Cross-reactivity with mouse and rat makes this antibody suitable for validation in translational models; confirm specificity in the relevant tissue or cell context using knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement with a comprehensive portfolio that includes validated antibodies, antigen pairs, and ancillary reagents. Our KATNB1 polyclonal antibody supports cytoskeletal and cell division research, areas vital for neuroscience and oncology diagnostics. Leveraging global supply networks, we offer bulk quantities and customized sourcing to meet your development needs from concept to clinic.