Anti-KAT8/MYST1/MOF Rabbit Polyclonal Antibody for WB, ELISA - Q9H7Z6

Core Product Specifications and Parameters

Biological Background: KAT8/MYST1/MOF Function and Localization

• Histone acetyltransferase KAT8 (also designated MYST1, hMOF) is a member of the MYST family of acetyltransferases, characterized by a conserved MYST domain and a C2HC zinc-finger motif.
• KAT8 catalyzes histone H4 acetylation at 'Lys-5', 'Lys-8', or 'Lys-16' depending on the associated complex: as part of the MSL complex, it generates H4K16ac to prevent chromatin compaction, while the NSL complex directs H4K5ac and H4K8ac to transcription start sites to promote initiation. Related references: PMID:12397079, PMID:16227571, PMID:16543150, PMID:20018852, PMID:22547026, PMID:33657400
• The MSL complex-mediated H4K16ac mark is uniquely transmitted across generations and regulates fundamental processes including oogenesis, embryonic stem cell pluripotency, hematopoiesis, and glucose metabolism. Related references: PMID:12397079, PMID:16227571, PMID:16543150
• Beyond histones, KAT8 acts as an acetyltransferase for numerous non-histone substrates: the NSL complex acetylates TP53/p53 at Lys-120 and KDM1A/LSD1, influencing apoptosis and epithelial-mesenchymal transition; other reported targets include IRF3 (antiviral immunity), PAX7 (muscle stem cell division), COX17 (mitochondrial integrity), and LMNA (nuclear architecture), with some activities inferred by similarity. Related references: PMID:17189187, PMID:19854137, PMID:27292636
• KAT8 localizes to the nucleus, chromosomes, and mitochondria. In mitochondria, it associates with mitochondrial DNA and controls respiratory gene expression in an acetyltransferase-dependent manner. Related references: PMID:27768893
• KAT8 also possesses protein propionyltransferase activity, further expanding its regulatory capacity. Related references: PMID:29321206
• The protein contains a Zn-finger domain and is subject to phosphorylation and acetylation, as indicated by proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1–200) of human KAT8, which encompasses part of the Zn-finger domain; antibodies generated against this region are expected to recognize full-length KAT8 and possibly splice variants retaining this sequence.
• For Western blot analysis, a predominant band around 52 kDa is anticipated, but consider potential band shifts due to post-translational modifications or variant isoforms; validate in cell lines known to express KAT8 (e.g., HeLa, HEK293).
• This polyclonal antibody is suitable for ELISA under non-denaturing conditions; consider its use in capture or detection formats after appropriate pairing and optimization.
• Cross-reactivity with mouse and rat is reported, enabling studies in these model organisms; however, confirm reactivity in your specific experimental system.
• For reliable results, perform pilot dilution experiments and include knockdown or knockout controls when feasible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive one-stop sourcing solution for IVD raw materials. This anti-KAT8 polyclonal antibody supports epigenetics and chromatin biology projects, complementing our extensive portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk quantities, rare target antibodies, or technical consultation, CamelBio bridges your needs from concept to clinic.