Anti-KAT5/Tip60 Polyclonal Antibody for WB, IF/ICC, ELISA - Q92993

Core Product Specifications and Parameters

Biological Background: KAT5/Tip60 Function and Localization

• Catalytic subunit of the NuA4 histone acetyltransferase complex that acetylates histones H2A and H4, promoting transcriptional activation, DNA repair, apoptosis, and cell cycle regulation. Related references: PMID:12776177, PMID:14966270, PMID:15042092, PMID:17709392, PMID:19783983, PMID:32832608
• Essential for double-strand break repair by homologous recombination: acetylates H2A at Lys-15 (H2AK15ac) to block 53BP1 recruitment and acetylates H2AX at Lys-5 to promote NBN assembly. Related references: PMID:27153538, PMID:32832608, PMID:17709392, PMID:26438602
• Acetylates non-histone substrates including ATM, p53, FOXP3, BMAL1, ULK1, RAN, NDC80, and AURKB, linking its activity to DNA damage signaling, autophagy, mitosis, and circadian rhythms. Related references: PMID:16141325, PMID:17360565, PMID:24835996, PMID:30704899, PMID:29040603, PMID:30409912, PMID:26829474
• In addition to acetylation, KAT5 can catalyze protein crotonylation, lactylation, and 2‑hydroxyisobutyrylation, adding layers of post‑translational regulation in mitosis and DNA repair. Related references: PMID:29192674, PMID:34608293, PMID:38961290
• During mitosis, KAT5 localizes to centromeres and kinetochores, where it acetylates AURKB, RAN, and NDC80 to ensure accurate chromosome segregation and microtubule attachment. Related references: PMID:26829474, PMID:29040603, PMID:30409912
• Localizes to the nucleus, chromosomes, centromeres, spindle poles, and cytoplasm, consistent with its multifunctional roles in transcription, repair, and cell division.
• Contains a zinc finger domain and is subject to phosphorylation, acetylation, and ubiquitination; alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody is raised against the N‑terminal region (aa 1‑230) of human KAT5, which is conserved across human, mouse, and rat; expect broad reactivity. Validate in the relevant sample system.
• For Western blot, a band near 59 kDa is anticipated; endogenous expression levels may vary among cell lines and tissues, so consider using positive controls such as HeLa or Jurkat lysates.
• In immunofluorescence/ICC, nuclear and/or cytoplasmic staining patterns are consistent with reported localizations; validate with appropriate fixation and permeabilization methods for your sample.
• The antibody has been tested in ELISA; for quantitative assays, perform titration and include proper negative controls.
• Since KAT5 undergoes multiple post‑translational modifications, the detected molecular weight may shift depending on treatment conditions; consider phosphatase inhibitors if phosphorylation‑dependent band shifts are of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For studies on chromatin regulation and DNA damage repair, this anti‑KAT5 antibody complements our range of histone modification research tools. Whether you need scalable monoclonal antibodies, bulk polyclonal reagents, or customized antigen production, our streamlined sourcing and technical support help accelerate your assay development from concept to clinic.