Anti-KAT2B/PCAF Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - Q92831

Core Product Specifications and Parameters

Biological Background: Histone acetyltransferase KAT2B Function and Localization

• KAT2B (PCAF) is a histone acetyltransferase that acetylates core histones H3 and H4, with strong preference for H3K9ac, to promote transcriptional activation. Related references: PMID:8945521 PMID:21131905
• It acetylates non-histone substrates including ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K, and TBX5, regulating mitosis, pre-rRNA processing, centrosome amplification, and heart/limb development. Related references: PMID:10675335 PMID:23001180 PMID:23932781 PMID:26867678 PMID:27796307 PMID:29174768
• KAT2B inhibits cell-cycle progression and counteracts the mitogenic activity of adenoviral oncoprotein E1A. Related references: PMID:8684459
• It acts as a circadian transcriptional coactivator, enhancing the activity of CLOCK-BMAL1 and NPAS2-BMAL1 heterodimers. Related references: PMID:14645221
• In HIV-1 infection, KAT2B is recruited by viral Tat to regulate proviral transcription and chromatin remodeling.
• Subcellular localization includes nucleus, cytoplasm, centrosome, and cytoskeleton.
• Ubiquitously expressed with highest levels in heart and skeletal muscle; also detected in skin keratinocytes. Related references: PMID:20940255
• Contains a bromodomain and also acetylates spermidine. Related references: PMID:27389534

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 360–450 within the N-terminal region of human KAT2B; consider that this may detect full-length and relevant isoforms.
• For Western blotting, validate the observed band against the predicted molecular weight of ~93 kDa in appropriate lysates, and include loading controls and negative controls.
• In immunoprecipitation studies, titrate the antibody to optimize capture efficiency and confirm specificity with isotype controls.
• For IHC-P and ELISA, preliminary optimization of antigen retrieval and detection conditions is recommended in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw materials, offering this anti-KAT2B/PCAF monoclonal antibody alongside validated antibody pairs, recombinant proteins, and ancillary reagents for histone modification and transcription research. Our supply chain supports diagnostic manufacturers with bulk quantities, technical consultation, and access to rare-target raw materials, streamlining development from concept to clinic.