Anti-KAT13A / SRC1 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q15788

Core Product Specifications and Parameters

Biological Background: KAT13A/SRC1 Function and Localization

• Encoded by the NCOA1 gene, KAT13A/SRC1 (also known as bHLHe74, RIP160) is a nuclear receptor coactivator and histone acetyltransferase.
• It directly binds and coactivates multiple nuclear receptors (e.g., PGR, GR, ER, RXRs, TRs, PPARs) in a hormone-dependent manner, stimulating transcription.
• Also serves as a coactivator for STAT3, STAT5A, STAT5B, and STAT6 transcription factors, linking cytokine signaling to gene expression.
• Possesses histone acetyltransferase (HAT) activity toward H3 and H4, potentially contributing to chromatin remodeling and transcription activation.
• Forms multisubunit coactivator complexes that participate in chromatin remodeling and recruitment of general transcription factors.
• Together with NCOA2, plays a role in controlling energy balance between white and brown adipose tissues.
• Isoforms 1, 2, and 3 differ in activity; isoform 2 exhibits the highest thyroid hormone-dependent transactivation.
• Located predominantly in the nucleus and widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400-750 of human KAT13A/SRC1; verify that this region is conserved in your target species and isoform of interest.
• For Western blot, a band at ~157 kDa is expected; consider using a low-percentage SDS-PAGE gel (e.g., 6–8%) and optimize transfer conditions for high-molecular-weight proteins.
• In immunoprecipitation experiments, validate antibody performance under both native and denaturing conditions, as the target may be part of large protein complexes.
• When setting up ELISA, the recombinant protein used as immunogen can serve as a positive control; however, pairing with capture or detection antibodies may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and laboratories from concept to clinic. For KAT13A/SRC1 research in nuclear receptor signaling and transcriptional regulation, we provide validated antibody pairs and optimized monoclonal antibodies, along with bulk ancillary reagents and rare target raw-material sourcing to accelerate your assay development.