Anti-Karyopherin β3 (IPO5) Monoclonal Antibody for WB, ELISA - O00410

Core Product Specifications and Parameters

Biological Background: Karyopherin β3 (IPO5) Function and Localization

• Importin-5 (IPO5, also known as karyopherin β3, RanBP5) is a member of the importin beta family of nuclear transport receptors that mediate the energy-dependent translocation of cargo proteins through the nuclear pore complex.
• It functions as a receptor for nuclear localization signals (NLS) in cargo substrates, facilitating the docking of the importin/substrate complex to nucleoporins and subsequent RanGTP-dependent release into the nucleoplasm.
• IPO5 specifically mediates the nuclear import of ribosomal proteins such as RPL23A, RPS7, and RPL5. Related references: PMID:11682607 PMID:9687515
• In vitro, IPO5 mediates the nuclear import of core histones H2A, H2B, H3, and H4.
• It is also involved in the nuclear import of CPEB3 following neuronal stimulation and has been hijacked by HIV-1 to import the viral Rev protein.
• Subcellular localization is predominantly cytoplasmic and nuclear, with presence in the nucleolus, consistent with its role in ribosomal protein transport.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity, and the protein undergoes alternative splicing.
• The canonical isoform has a calculated molecular weight of approximately 123.6 kDa, aligning with the observed ~124 kDa band in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 20-300) of IPO5, which is well-conserved among human, mouse, and rat, supporting the observed cross-reactivity; consider sequence alignment for other species.
• For Western blot, a band around 124 kDa is expected; optimize loading and transfer conditions for high-molecular-weight proteins.
• In ELISA, the antibody can be used to detect endogenous or recombinant IPO5; validate coating and blocking conditions in your specific assay format.
• Consider testing in immunoprecipitation or immunofluorescence if applications beyond WB/ELISA are required, though validation is needed.

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