Anti-KAP1/TRIM28 Polyclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - Q13263

Core Product Specifications and Parameters

Biological Background: KAP1/TRIM28 Function and Localization

• Also known as transcription intermediary factor 1-beta (TIF1-beta), KRAB-associated protein 1 (KAP-1), or KRIP-1, this 88.6 kDa tripartite motif (TRIM) family protein features RING, B-box, coiled-coil, PHD, and bromodomain regions.
• Functions as an E3 SUMO and ubiquitin ligase that scaffolds transcriptional repression complexes by recruiting SETDB1 (histone methyltransferase) and NuRD (nucleosome remodeling and deacetylation) complex, leading to H3K9 methylation and heterochromatin formation.
• Serves as a nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs), mediating silencing of endogenous retroviruses and genes critical for embryonic development and genomic imprinting.
• In regulatory T-cells, cooperates with ZFP90 isoform 2 to potentiate the transcriptional repressor activity of FOXP3, essential for Treg suppressive function. Related references: PMID:23543754
• Maintains a transcriptionally repressive state in undifferentiated embryonic stem cells (ESCs), preserving pluripotency and early developmental potential. Related references: PMID:24623306
• Acts as a positive regulator of type I interferon (IFN-I) signaling by mediating Lys-63-linked ubiquitination of TBK1, facilitating TBK1-IRF3 complex formation. Related references: PMID:38495890
• Regulates innate immunity and inflammation through SUMOylation of key mediators: TRAF6 SUMOylation controls nucleo-cytoplasmic shuttling and NF-kappa-B activation, while NLRP3 SUMOylation stabilizes the inflammasome and enhances activation. Related references: PMID:39920527, PMID:34373456
• Localizes predominantly to the nucleus and is broadly expressed across human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 576-835) containing the PHD and bromodomain; the resulting polyclonal antibody may recognize full-length KAP1/TRIM28 and potential splice variants or post-translationally modified forms.
• For Western blotting, a band around 89 kDa (predicted 88.6 kDa) is expected; consider using 8–10% SDS-PAGE for optimal resolution and validate in lysates from human, mouse, or rat cells.
• In immunoprecipitation assays, optimize antibody concentration and lysis buffer conditions; crosslinking the antibody to beads may improve capture and reduce heavy/light chain interference.
• For immunofluorescence/ICC, nuclear staining is anticipated; use appropriate fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) and include DAPI co-staining.
• When performing immunohistochemistry (IHC-P), antigen retrieval in citrate buffer (pH 6.0) is often beneficial; always include positive and negative tissue controls to confirm specificity.
• For ELISA applications, paired with a detection antibody, determine optimal coating antigen concentration and antibody dilutions empirically using known positive and negative samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering validated antibodies, matched antibody pairs, recombinant proteins, and ancillary reagents to streamline assay development. Our KAP1/TRIM28 product line supports research in transcriptional regulation, epigenetics, and immune signaling, with polyclonal and monoclonal options available in bulk. By leveraging our global supplier network, we expedite sourcing of rare-target reagents and deliver comprehensive technical support from concept to clinic.