Anti-KAP1/TRIM28 Monoclonal Antibody for WB, IHC-P, ELISA - Q13263

Core Product Specifications and Parameters

Biological Background: KAP1/TRIM28 Function and Localization

• Transcription intermediary factor 1-beta (TIF1β), also known as KAP1/TRIM28, is an E3 SUMO and ubiquitin ligase essential for embryonic development, genomic imprinting, and maintaining genomic stability by silencing repetitive and retroviral elements.
• Functions as a scaffold for transcriptional repression complexes, acting as a corepressor for KRAB-ZFPs by recruiting CHD3 (NuRD complex) and SETDB1 to establish H3K9me3 and heterochromatin.
• Collaborates with SETDB1 to silence endogenous and introduced retroviruses via H3K9me3 in a DNA-methylation independent pathway (By similarity).
• Plays a dual role as coactivator for CEBPB and NR3C1 and corepressor for ERBB4, and inhibits E2F1 activity by promoting E2F1-HDAC1 complex formation to suppress apoptosis.
• Essential for FOXP3-mediated transcriptional repression in regulatory T-cells (PubMed:23543754) and for maintaining the repressive state of genes in undifferentiated embryonic stem cells (PubMed:24623306). Related references: PMID:23543754 PMID:24623306
• Positive regulator of innate immunity: promotes TBK1 ubiquitination in IFN-I signaling (PubMed:38495890) and mediates TRAF6 SUMOylation to regulate NF-κB (PubMed:39920527). Related references: PMID:38495890 PMID:39920527
• Enhances NLRP3 inflammasome activation via SUMOylation (PubMed:34373456). Related references: PMID:34373456
• Subject to multiple post-translational modifications including acetylation, phosphorylation, SUMOylation, and ADP-ribosylation, which regulate its activity and interactions.
• Subcellular localization: nucleus. Widely expressed in many tissues including spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The immunogen lies within the central region (aa 250-350) of human KAP1/TRIM28, which is conserved across human, mouse, and rat, supporting broad species reactivity.
• For Western blot (WB), the predicted molecular weight is ~89 kDa. Consider using 8-10% SDS-PAGE and validate with appropriate loading controls in your specific cell or tissue lysates.
• For IHC-P, antigen retrieval may be required; citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) are common starting points. Always include known positive and negative tissue controls.
• For ELISA, the antibody may be used as capture or detection reagent; optimal coating concentrations and pairings should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio provides validated antibodies, antigens, and ancillary reagents to support diagnostic development. This anti-KAP1/TRIM28 monoclonal antibody is ideal for chromatin biology, epigenetics, and immuno-oncology research applications. For bulk orders, custom antibody pairs, or rare-target sourcing, please contact our technical support team.