Anti-KANSL3 Rabbit Polyclonal Antibody for WB and ELISA - Q9P2N6

Core Product Specifications and Parameters

Biological Background: KANSL3 Function and Localization

• KANSL3 (also known as NSL3, Rcd1, KIAA1310) is a non-catalytic component of the NSL histone acetyltransferase complex that mediates histone H4 acetylation at Lys-5 and Lys-8 (H4K5ac and H4K8ac) at transcription start sites, thereby promoting transcription initiation. Related references: PMID:20018852, PMID:33657400
• Within the NSL complex, KANSL3 is required for KAT8 association with mitochondrial DNA and functions as a regulator of mitochondrial gene expression. Related references: PMID:27768893
• KANSL3 acts as a microtubule minus-end binding protein that stabilizes microtubules and promotes their assembly, playing an essential role in spindle assembly during mitosis. Related references: PMID:26243146
• It is required for transcription of intraciliary transport genes, necessary for cilium assembly in ciliated cells and for organization of the microtubule cytoskeleton in non-ciliated cells (By similarity).
• KANSL3 maintains nuclear architecture stability by promoting KAT8-mediated acetylation of lamin LMNA (By similarity).
• The protein localizes to the nucleus, mitochondrion, cytoplasm, and spindle poles, consistent with its diverse roles.
• KANSL3 is phosphorylated and undergoes alternative splicing, producing multiple isoforms. It is also known as testis development protein PRTD, highlighting its importance in early embryonic development and lineage specification.

Experimental Guidance and Technical Tips

• Consider using this antibody for Western blotting to detect KANSL3 in human cell lysates; the predicted molecular weight is approximately 96 kDa.
• For ELISA, validate with the recombinant immunogen (amino acids 1-200) or native protein to confirm reactivity.
• Due to the protein's multi-compartment localization, subcellular fractionation may enhance detection sensitivity in Western blot.
• Note that alternative splicing may result in multiple bands; compare band patterns against known isoforms in appropriate databases.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating chromatin regulation, microtubule biology, or developmental processes, CamelBio supplies validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents for assay development. Our extensive sourcing capabilities extend to rare targets, ensuring you have the critical tools needed to bridge discovery and clinical application efficiently.