Anti-Kallistatin (SERPINA4) Rabbit Polyclonal Antibody for WB - P29622

Core Product Specifications and Parameters

Biological Background: Kallistatin Function and Localization

• Kallistatin, also known as Serpin A4, peptidase inhibitor 4 (PI-4), or kallikrein inhibitor, is a member of the serine protease inhibitor (serpin) superfamily encoded by the SERPINA4 gene.
• It functions as a specific inhibitor of tissue kallikrein, blocking its amidolytic and kininogenase activities. This inhibition involves the formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme.
• During inhibition, the reactive site loop of kallistatin is cleaved by tissue kallikrein, generating a small C-terminal fragment. This cleavage is a typical mechanism for serpin-mediated protease inhibition.
• Kallistatin is a secreted glycoprotein, as indicated by its subcellular localization (secreted) and keyword annotation (glycoprotein, signal peptide).
• The protein is predominantly expressed by the liver and secreted into plasma, consistent with its role in regulating circulating kallikrein activity.
• Keywords highlight its classification as a serine protease inhibitor, glycoprotein, and secreted protein, with experimental evidence from direct protein sequencing and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 178–427) covers a large internal region of the protein, likely recognizing multiple epitopes. This design may provide robust detection in denaturing and native conditions.
• For Western blot, expect a band around 49 kDa (as indicated) in samples expressing SERPINA4, such as liver tissue or plasma. Validate specificity using positive controls and appropriate blocking.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider that kallistatin is secreted, so intracellular staining may be limited. Optimize fixation and permeabilization to detect cytoplasmic pools or secretory pathway intermediates.
• For ELISA applications, this polyclonal antibody can be used as either capture or detection reagent after empirical optimization of working concentrations and pairing with suitable secondary antibodies.
• Cross-reactivity with mouse and rat suggests utility in rodent models; however, validate species-specific performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Kallistatin polyclonal antibody exemplifies our capability to supply quality reagents for serpin/protease inhibitor research, and we can assist with customized sourcing or scaling for your assay development needs.