Anti-Kallikrein 8 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O60259

Core Product Specifications and Parameters

Biological Background: Kallikrein-8 Function and Localization

• Kallikrein-8 (Neuropsin) is a serine protease belonging to the tissue kallikrein family, encoded by the KLK8 gene. It exists in multiple isoforms generated by alternative splicing.
• It cleaves substrates such as L1CAM in response to neural activity, promotes neurite outgrowth, and modulates synaptic plasticity, thereby playing a critical role in memory acquisition and hippocampal function.
• The protease also degrades extracellular matrix components including fibronectin, collagen type IV, and fibrinogen, implicating it in tissue remodeling and cancer progression.
• Subcellular localization includes secretion into the extracellular space and cytoplasmic presence, consistent with its roles in both intercellular signaling and intracellular processing.
• Tissue expression is diverse: isoform 1 is abundant in pancreas, isoform 2 is brain-specific, and both are found in fetal tissues. Overexpression is associated with Alzheimer's disease, ovarian carcinoma, and inflammatory skin disorders like psoriasis.
• Key post‑translational features include glycosylation, disulfide bond formation, and activation from a zymogen precursor, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the 161–260 region of human Kallikrein‑8. This linear epitope design is suitable for Western blot under denaturing conditions.
• For IHC‑P, consider the need for antigen retrieval and validate staining in positive (e.g., skin or brain) and negative control tissues to confirm specificity.
• In ELISA, the peptide immunogen can serve as a positive control to calibrate binding. Ensure that the assay conditions (e.g., coating buffer, blocking agent) are optimized for monoclonal antibodies.
• Because Kallikrein‑8 is both secreted and cytoplasmic, fractionation or specific extraction protocols may be required depending on the target localization in your samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and researchers a streamlined gateway to premium IVD raw materials. For studies involving Kallikrein‑8 in neurodegeneration, cancer, or dermatology, we can supply validated antibody pairs, high‑affinity monoclonal/polyclonal antibodies, and complementary reagents in bulk. Our sourcing network and technical support help you seamlessly scale from discovery to clinical application.