Anti-Kallikrein 10 (NES1/PRSSL1) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O43240

Core Product Specifications and Parameters

Biological Background: Kallikrein 10 Function and Localization

• Kallikrein-10 (KLK10), also known as normal epithelial cell-specific 1 (NES1) and protease serine-like 1 (PRSSL1), is a secreted serine protease of the kallikrein family.
• It functions as a tumor suppressor in breast and prostate cancer, inhibiting cell proliferation and promoting apoptosis.
• The protein is primarily expressed in glandular tissues, including breast, ovary, and prostate, where it plays a role in epithelial cell homeostasis.
• Subcellularly, KLK10 is secreted into the extracellular space, consistent with its role as a serine hydrolase involved in proteolytic cascades.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are critical for its structural stability and enzymatic activity.
• The protein is implicated in cell cycle regulation, and its expression is often downregulated in malignant tissues, highlighting its tumor-suppressive properties.
• As a serine protease, KLK10 may participate in zymogen activation and extracellular matrix remodeling, though its specific natural substrates remain under investigation.
• The UniProt keyword "3D-structure" indicates that its three-dimensional structure has been solved, which may aid in antibody design and functional studies.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 43-276 of human KLK10 (NP_665895.1), which encompasses a large portion of the extracellular protease domain; therefore, the antibody is likely to recognize the mature secreted protein.
• For Western blot (WB) applications, consider using a dilution range of 1:500–1:2000 and validate with a positive control such as recombinant KLK10 protein or cell lysates from breast, ovarian, or prostate cancer lines expressing KLK10.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize permeabilization and blocking steps; note that KLK10 is secreted, so fixation may require specific conditions to retain the protein in the pericellular space.
• For ELISA, the antibody can be used as a capture or detection antibody; however, pairing with another clone against a distinct epitope is recommended for sandwich ELISA development. Validate with purified antigen or clinical samples to assess performance.
• As a rabbit monoclonal antibody, it offers high lot-to-lot consistency and specificity, making it suitable for long-term studies or diagnostic assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-KLK10 product, bulk ancillary reagents, and rare target raw-material sourcing. For research on breast, ovarian, and prostate cancers, CamelBio supports your workflow from early-stage biomarker discovery to immunoassay development. Contact us to explore customized procurement options and technical consulting tailored to your project needs.