Anti-Kaiso/ZBTB33 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q86T24

Core Product Specifications and Parameters

Biological Background: Kaiso/ZBTB33 Function and Localization

• Transcriptional regulator Kaiso, also known as Zinc finger and BTB domain-containing protein 33 (ZBTB33), encoded by the ZBTB33 gene (gene ID 10009).
• Exhibits bimodal DNA-binding specificity: binds to methylated CpG dinucleotides (consensus 5'-CGCG-3') and the non-methylated consensus sequence 5'-CTGCNA-3' (kaiso binding site, KBS).
• Recruits the N-CoR repressor complex to promote histone deacetylation and formation of repressive chromatin structures at target gene promoters, thereby regulating transcription.
• May contribute to the repression of target genes of the Wnt signaling pathway, and can also activate transcription of a subset of target genes by recruiting CTNND2.
• Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2, and RUNX1T1. Related references: PMID:23251453
• Subcellular localization is primarily nuclear but also detected in the cytoplasm.
• Tissue specificity: expressed in vascular endothelium.
• Post-translational modifications and functional annotations include phosphorylation, ubiquitination, metal-binding (zinc), and roles as both activator and repressor (UniProt keywords: Phosphoprotein, Isopeptide bond, Ubl conjugation, Zinc-finger).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 573-672 of human Kaiso (Q86T24), located in the C-terminal region, which suggests the antibody may recognize epitopes within this domain.
• For Western blot (WB), a band at approximately 74 kDa (predicted molecular weight ~74.5 kDa) is expected; use appropriate molecular weight markers.
• In immunofluorescence (IF/ICC), anticipate nuclear and possibly cytoplasmic staining; optimize fixation and permeabilization conditions for your cell type.
• The antibody has been shown to cross-react with human and rat samples; for other species, validate homology of the immunogen sequence to avoid non-specific binding.
• For IP and ELISA applications, determine optimal antibody concentration by titration in your specific experimental setup.

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