Anti-Kaiso/ZBTB33 Polyclonal Antibody for WB, IHC-P, ELISA - Q86T24

Core Product Specifications and Parameters

Biological Background: Kaiso Function and Localization

• Also known as zinc finger and BTB domain-containing protein 33 (ZNF348/ZNF-kaiso), Kaiso is a transcriptional regulator encoded by the ZBTB33 gene in human.
• Functions as a bimodal DNA-binding protein: it recognizes methylated CpG dinucleotides in the consensus 5'-CGCG-3' sequence and the non-methylated kaiso binding site (KBS) 5'-CTGCNA-3'.
• Recruits the N-CoR repressor complex to gene promoters, promoting histone deacetylation and formation of repressive chromatin structures to silence target genes of the Wnt signaling pathway.
• May also activate transcription of certain target genes by recruiting CTNND2, demonstrating context-dependent regulatory duality.
• Represses MMP7 expression through cooperation with corepressors CBFA2T3, CBFA2T2 and RUNX1T1. Related references: PMID:23251453
• Primarily localizes to the nucleus, with cytoplasmic distribution also observed, consistent with its roles in transcriptional regulation.
• Expressed in vascular endothelium and implicated in pathways involving DNA binding, metal (zinc) coordination, and post-translational modifications such as phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 523–672 of human Kaiso (NP_006768.1). Verify specificity using appropriate positive and negative controls.
• For Western blotting, the predicted molecular weight is approximately 74 kDa; select gel percentage and transfer conditions accordingly.
• In immunohistochemistry (paraffin), validate staining patterns in known positive tissues (e.g., vascular endothelium) and include isotype controls to confirm nuclear or cytoplasmic signals.
• Cross-reactivity is reported for human and mouse; when working with other species, perform sequence alignment and experimental validation.
• Given the dual repressor/activator function, interpret results in the context of the Wnt pathway or specific cofactors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined, one-stop sourcing solution for IVD raw materials, technical services, and consulting—supporting diagnostic manufacturers from concept to clinic. This Kaiso/ZBTB33 polyclonal antibody serves researchers exploring transcriptional regulation, Wnt signaling, and cancer biology. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or emerging targets to accelerate assay development and production scaling.