Anti-K-Cadherin (CDH6) Rabbit Monoclonal Antibody for WB, ELISA - P55285

Core Product Specifications and Parameters

Biological Background: K-Cadherin (CDH6) Function and Localization

• Cadherin-6 (CDH6), also known as K-cadherin, is a member of the cadherin superfamily of calcium-dependent cell adhesion proteins.
• It mediates homophilic cell-cell adhesion, contributing to the sorting of heterogeneous cell types and tissue morphogenesis.
• CDH6 is a single-pass type I transmembrane protein localized primarily at the cell membrane.
• The human CDH6 gene produces multiple isoforms through alternative splicing.
• It is highly expressed in brain, cerebellum, and kidney, with weaker expression in lung, pancreas, and gastric mucosa; also detected in certain liver and kidney carcinomas.
• Post-translational modifications include extracellular Ca2+-binding for adhesive function, potential cleavage on pair of basic residues, and phosphorylation, as indicated by keywords.
• The protein contains cadherin repeat domains that mediate homophilic interactions in a Ca2+-dependent manner.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (amino acids 691–790) of human CDH6, which may be suitable for detecting total CDH6 regardless of cleavage.
• When using this antibody in Western blot (WB), consider a predicted molecular weight of approximately 88 kDa, though observed bands may vary depending on sample preparation and post-translational modifications.
• Validate antibody specificity in the relevant sample system, using appropriate positive and negative controls.
• For ELISA applications, optimize coating and detection conditions to achieve the desired sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. The K-Cadherin (CDH6) antibody targets an essential cell adhesion molecule relevant to kidney, brain, and cancer research. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your workflow development.