Anti-JunD Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17535

Core Product Specifications and Parameters

Biological Background: JunD Function and Localization

• JunD, also known as transcription factor AP-1 subunit JunD, is a member of the Jun family of transcription factors.
• It binds to AP-1 consensus sequences (5'-TGA[GC]TCA-3') to regulate gene expression.
  Related references: PMID:9989505
• JunD forms heterodimers with FOS family proteins, enhancing DNA binding and transcriptional activity.
  Related references: PMID:28981703, PMID:9989505
• The protein localizes primarily to the nucleus, consistent with its role as a transcription regulator.
• Post-translational modifications include phosphorylation and disulfide bond formation, which may influence its structure as noted by UniProt keywords.
• JunD plays roles in cell proliferation, differentiation, and apoptosis as part of the AP-1 complex.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-190 of human JunD, targeting the N-terminal region. This region is conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blot (WB), consider using whole cell lysates and optimize antibody dilution for your specific sample and detection system.
• Immunohistochemistry (IHC-P) may require antigen retrieval; pilot experiments with known positive control tissues are recommended.
• ELISA can be used for protein quantification; include appropriate standards and negative controls.
• As a polyclonal antibody, it may recognize multiple epitopes, potentially offering robust signal but requiring careful validation for specificity in the target application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, providing high-quality antibodies like this anti-JunD polyclonal for transcription factor research. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic.