Anti-JunD Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P17535

Core Product Specifications and Parameters

Biological Background: JunD Function and Localization

• JunD (AP-1) is a transcription factor encoded by the JUND gene and belongs to the Jun family of AP-1 subunits.
• Binds to AP-1 consensus sites (3'-TGA[GC]TCA-5') as a homodimer or heterodimer with FOS family proteins, enhancing DNA-binding and transcriptional activity.
  Related references: PMID:9989505, PMID:28981703
• Localizes to the nucleus, consistent with its role in transcription regulation.
• Annotated as a phosphoprotein and contains a disulfide bond (by UniProt keyword), suggesting regulation via phosphorylation and redox state.
• Has 3D structure data available (UniProt keyword '3D-structure'), indicating solved protein structures.
• Identified in proteomics studies (keyword 'Proteomics identification') and is part of the reference human proteome.
• Functional keywords: activator, DNA-binding, transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-141 of human JunD) targets the N-terminal region and may recognize the full-length protein under denaturing and native conditions. Consider validating in relevant sample systems expressing JunD, such as human, mouse, and rat cell lines.
• For Western blotting (WB), a band around 35 kDa is expected. Optimize antibody dilution and incubation conditions empirically.
• Suitable for immunohistochemistry (IHC-P), immunoprecipitation (IP), and ELISA. Verify compatibility with your specific protocol and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with end-to-end IVD raw-material procurement and technical services. For transcription factor and AP-1 pathway studies, we provide validated monoclonal antibodies like this JunD rabbit mAb, optimized antibody pairs, and bulk ancillary reagents. Streamline your supply chain with CamelBio’s single-source access to rare targets and expert consulting.