Anti-JunB Rabbit Polyclonal Antibody for WB, ELISA - P17275

Core Product Specifications and Parameters

Biological Background: JunB Function and Localization

• JunB is a transcription factor and a key component of the AP-1 complex.
• It heterodimerizes with FOS family proteins to bind the DNA consensus sequence 5'-TGA[GC]TCA-3'.
• Functions in regulating gene expression in response to growth factor signaling.
• Primarily localized in the nucleus.
• Subject to multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination.
• The protein has a predicted molecular weight of ~35.9 kDa.
• Encoded by the JUNB gene in humans.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–200) of human JunB; thus, the antibody is likely to recognize epitopes within this domain.
• For Western blot, use standard sample preparation and transfer conditions. An expected band can be observed at approximately 36 kDa. Optimize primary antibody dilution and incubation conditions based on the experimental system.
• For ELISA, the antibody may be employed as a detection or capture reagent; optimal coating concentrations and working dilutions should be determined empirically.
• Validate antibody specificity using appropriate controls, such as JunB knockdown or knockout cell lysates, or by peptide blocking assay.
• This antibody is reactive with human JunB; reactivity with other species has not been tested and should be verified if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering a wide range of high-quality antibodies like this anti-JunB polyclonal antibody to advance transcription factor research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development. For rare or custom targets, our sourcing network provides flexible solutions from concept to clinic.