Anti-JunB Polyclonal Antibody for WB, IF/ICC, ELISA - P17275

Core Product Specifications and Parameters

Biological Background: JunB Function and Localization

• JunB is a member of the Jun family of transcription factors, which are part of the AP-1 complex; it is encoded by the JUNB gene in humans and is also known as AP-1 transcription factor subunit JunB.
• It functions as a transcription factor that regulates gene activity in response to primary growth factor signals by binding to the DNA consensus sequence 5'-TGA[GC]TCA-3'.
• JunB can heterodimerize with proteins of the FOS family to form the AP-1 transcription complex, which enhances its DNA binding affinity and transcriptional activity.
• Subcellular localization studies indicate that JunB is predominantly found in the nucleus, consistent with its role in transcriptional regulation.
• The protein undergoes various post-translational modifications, including acetylation, phosphorylation, and ubiquitin conjugation, which may modulate its activity and stability.
• Key biological processes associated with JunB include transcription regulation, cell proliferation, differentiation, and apoptosis, as inferred from its role in the AP-1 complex.

Experimental Guidance and Technical Tips

• Given the immunogen design covering amino acids 1-240, which includes critical functional domains, users can expect recognition of full-length JunB (predicted MW ~36 kDa) in Western blot assays; consider using nuclear extracts for optimal detection.
• For immunofluorescence/immunocytochemistry (IF/ICC), validate the antibody’s nuclear staining pattern in positive control cells, as JunB localizes to the nucleus.
• In ELISA applications, a recombinant JunB protein (e.g., immunogen sequence) can serve as a positive control to establish the standard curve; cross-reactivity with mouse and rat JunB may allow multispecies assay development.
• As a polyclonal antibody, performance may vary across batches; it is advisable to titrate the antibody for each new lot and application, and to include appropriate positive and negative controls.

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CamelBio supplies high-quality IVD raw materials, such as this JunB polyclonal antibody, to diagnostic manufacturers and research laboratories worldwide. Our one-stop service covers rapid procurement of bulk antibodies, validated antibody pairs, recombinant proteins, and ancillary reagents, enabling streamlined kit development from concept to clinic. For rare or custom targets, our sourcing experts provide tailored solutions to meet your assay-specific needs.