Anti-JunB Monoclonal Antibody for WB, IHC-P, ELISA - P17275

Core Product Specifications and Parameters

Biological Background: Transcription factor JunB Function and Localization

• Transcription factor JunB is a component of the AP-1 complex, which regulates gene expression in response to various stimuli, including growth factors.
• It binds to the consensus DNA sequence 5'-TGA[GC]TCA-3' and forms heterodimers with FOS family proteins to enhance transcriptional activity.
• Encoded by the JUNB gene, it belongs to the JUN family of transcription factors.
• JunB is localized to the nucleus, consistent with its role in transcriptional regulation.
• Key post-translational modifications include acetylation and phosphorylation, which may modulate its activity.
• It is a reference proteome member and has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blot, consider using a positive control such as HeLa or Jurkat whole cell lysates, as JunB is expressed in various human cell lines.
• For IHC-P, antigen retrieval may be necessary; optimize antibody dilution using a known positive tissue.
• ELISA: The synthetic peptide immunogen can be used as a coating antigen for direct ELISA validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing platform for IVD raw materials. This anti-JunB antibody supports research into AP-1 transcription factor biology, gene regulation, and cancer-related signaling. Beyond recombinant antibodies, we supply validated antibody pairs, auxiliary reagents, and customized bulk sourcing for rare targets.