Anti-JTB Polyclonal Antibody for WB and ELISA - O76095

Core Product Specifications and Parameters

Biological Background: JTB Function and Localization

• Protein JTB (Jumping translocation breakpoint protein), also known as PAR or HSPC222, is encoded by the JTB gene; alternative splicing yields multiple isoforms.
• Plays a critical role in cytokinesis and the regulation of cell proliferation; essential for normal mitotic progression.
• Likely a component of the chromosomal passenger complex (CPC), which governs chromosome alignment, segregation, and spindle assembly; enhances AURKB kinase activity.
• Inhibits TGFB1-dependent apoptosis; overexpression leads to mitochondrial swelling and loss of membrane potential.
• Subcellular localization includes the membrane, mitochondrion, cytoplasm, centrosome, and mitotic spindle.
• Ubiquitously expressed in normal human tissues, but expression is frequently altered in malignant counterparts, underscoring its relevance in cancer research.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 31–105, a segment conserved in rat, rationalizing the observed cross-reactivity.
• For western blotting, anticipate a band near 16 kDa under reducing conditions; validate detection in your specific lysate.
• ELISA reactivity indicates potential for quantitative applications; confirm performance with appropriate antigen standards and controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier offering validated polyclonal antibodies, such as this anti-JTB antibody, to support cell cycle and oncology research. We provide one-stop access to optimized antibody pairs, recombinant proteins, and ancillary reagents, streamlining your diagnostic development from concept to clinic.