Anti-JPH4 Polyclonal Antibody for WB, ELISA - Q96JJ6

Core Product Specifications and Parameters

Biological Background: Junctophilin-4 Function and Localization

• Junctophilin-4 (JPH4) is a member of the junctophilin family and is also known as junctophilin-like 1 protein.
• JPH4 contributes to the formation of junctional membrane complexes (JMCs) that tether the plasma membrane to the endoplasmic or sarcoplasmic reticulum in excitable cells.
• It provides a structural foundation for functional cross-talk between cell surface and intracellular calcium release channels, essential for calcium signaling.
• JPH4 is specifically expressed in the brain and appears to play an active role in certain neurons involved in motor coordination and memory (By similarity).
• The protein localizes to both the cell membrane and the endoplasmic reticulum membrane.
• JPH4 contains transmembrane domains and repeat regions, consistent with its membrane-tethering function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 405-605) of human JPH4; consider using appropriate positive and negative controls in western blotting experiments.
• For WB, validate the antibody in relevant brain tissue lysates or neuronal cell lines from human, mouse, or rat, given the reported cross-reactivity.
• ELISA applications may require optimization of coating and detection conditions; preliminary checkerboard titration is advisable.
• Take into account the predicted molecular weight of ~66 kDa when assessing band specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing by offering validated antibodies, bulk ancillary reagents, and technical support—accelerating development in neuroscience and calcium-signaling research. This JPH4 polyclonal antibody, along with potential custom monoclonal antibody pairs and optimized reagents, can be procured to suit diverse assay development needs. As a dedicated B2B partner, CamelBio bridges the gap between concept and clinic, particularly for targets involved in junctional membrane complexes and neuronal function.