Anti-JPH1 Rabbit Polyclonal Antibody for WB - Q9HDC5

Core Product Specifications and Parameters

Biological Background: Junctophilin-1 Function and Localization

• Junctophilin-1 (JPH1) is a member of the junctophilin family, which contributes to the formation of junctional membrane complexes (JMCs) that physically link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells.
• JPH1 provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels, enabling efficient excitation-contraction coupling.
• In skeletal muscle, JPH1 specifically contributes to the construction of the triad junction by linking the transverse-tubule (t-tubule) and sarcoplasmic reticulum (SR) membranes.
• Subcellularly, JPH1 localizes to the cell membrane, endoplasmic reticulum membrane, and sarcoplasmic reticulum membrane, featuring transmembrane domains that anchor it at these contact sites.
• JPH1 is abundantly expressed in skeletal muscle, with very low expression in the heart, reflecting its specialized role in muscle triad organization.
• Genetic variants in JPH1 are associated with Charcot-Marie-Tooth disease type 2K (CMT2K), highlighting its clinical significance in neuromuscular disorders.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 410–640; consider verifying that this region is accessible in the native conformation of your sample.
• This polyclonal antibody is suitable for Western blot (WB) and ELISA detection of human JPH1 and cross-reacts with mouse, enabling multi-species study designs.
• For WB, the predicted molecular weight is approximately 72 kDa; select an appropriate gel percentage and transfer conditions to resolve this band clearly.
• Validate antibody specificity in skeletal muscle lysates (positive control) and non-muscle tissues or JPH1-knockout models (negative controls) to confirm target engagement.

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CamelBio offers diagnostic manufacturers and research laboratories a single point of access to IVD raw materials, including validated polyclonal antibodies such as this anti-JPH1 antibody for neuromuscular research. Our extensive portfolio covers monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support your workflow from early development to clinical application. Partner with us for streamlined procurement and technical support tailored to your project needs.