Anti-JOSD1 Polyclonal Antibody for WB, ELISA - Q15040

Core Product Specifications and Parameters

Biological Background: Josephin-1 Function and Localization

• JOSD1, also known as Josephin domain-containing protein 1, is a deubiquitinating enzyme that contains a Josephin catalytic domain.
• It can cleave both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains in vitro, suggesting roles in protein turnover and signaling.
• JOSD1 may regulate endocytosis by promoting macropinocytosis and suppressing clathrin- and caveolae-mediated pathways.
• The protein potentially enhances membrane dynamics and cell motility independently of its deubiquitinase activity.
• It localizes to both the cell membrane and cytoplasm, consistent with its role in membrane trafficking.
• JOSD1 is subject to phosphorylation and is implicated in ubiquitin conjugation pathways, as indicated by proteomics and phosphoproteomics data.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human JOSD1 protein (aa 1–202), suggesting the antibody may recognize the native protein in various assays.
• For Western blotting, expect a band around 23kDa; validate specificity using appropriate positive and negative controls.
• In ELISA applications, consider testing a range of antibody dilutions with recombinant protein or cell lysates.
• Cross-reactivity with mouse and rat allows for use in rodent models, but confirm detection efficacy in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. This anti-JOSD1 antibody fits into studies of deubiquitination and membrane dynamics, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your project. Leverage our sourcing network for rare target raw materials and accelerate your development from concept to clinic.