Anti-JNK3 Rabbit Polyclonal Antibody for WB, ELISA - P53779

Core Product Specifications and Parameters

Biological Background: JNK3 Function and Localization

• JNK3, encoded by the MAPK10 gene, is a serine/threonine-protein kinase belonging to the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) family. Alternative splicing generates multiple isoforms.
• Extracellular stimuli such as pro-inflammatory cytokines or physical stress activate the SAPK/JNK cascade, wherein dual-specificity kinases MAP2K4 and MAP2K7 phosphorylate and activate JNK3. Activated JNK3 then phosphorylates transcription factors like JUN and ATF2, thereby regulating AP-1-dependent transcription.
• JNK3 plays a critical role in neuronal apoptosis by phosphorylating substrates such as STMN2 and APP, influencing neuronal differentiation, migration, and neurite growth in spiral ganglion neurons.
• JNK3 contributes to circadian rhythm regulation by phosphorylating the CLOCK-BMAL1 heterodimer (PubMed:22441692), and also phosphorylates JUND, a process inhibited by MEN1 (PubMed:22327296).
  Related references:
  PMID:22441692
  PMID:22327296
• Shuttles between the cytoplasm, nucleus, mitochondria, and membrane, consistent with its roles in signal transduction and apoptosis.
• Expression is predominantly neuronal, with high levels in the hippocampus, cerebellum, striatum, and brain stem, and weak expression in testis and kidney.
• JNK3 undergoes palmitoylation and phosphorylation at multiple residues, which regulate its subcellular localization and kinase activity. Mutations in MAPK10 are associated with neurological disorders, including epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 300-400) lies within a conserved region shared with mouse and rat JNK3, enabling cross-species detection. For Western blot, validate the antibody in the relevant sample system, expecting a predominant band near 44 kDa (predicted weight may vary due to isoforms or modifications). Use neuronal cell lines or brain tissue homogenates as positive controls.
• For ELISA, the antibody can be used in sandwich or indirect formats; consider pairing with a monoclonal capture antibody for optimal sensitivity. JNK3 phosphorylation can be induced by stress stimuli (e.g., anisomycin, UV) to monitor activation-dependent signals.
• As a polyclonal antibody, it may recognize multiple isoforms or degradation products. Pre-adsorption with the immunogen peptide can be used to confirm specificity.

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